Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10487
Title: Molecular Detection of Leishmania in Sand Flies (Diptera: Psychodidae: Phlebotominae) Collected in the Caititu Indigenous Reserve of the Municipality of Lábrea, State of Amazonas, Brazil
Authors: Silva, Túlio Romão Ribeiro da
Assis, Mauro Diego Gobira Guimarães de
Freire, Maíra Posteraro
Rêgo, Felipe Dutra
Gontijo, Celia Maria Ferreira
Shimabukuro, Paloma Helena Fernandes
Affilliation: Universidade Federal do Amazonas. Programa de Pós-Graduaçâo em Saúde, Sociedade e Endemias na Amazônia. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brazil/Universidade Federal do Pará. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brazil
Universidade Federal do Amazonas. Programa de Pós-Graduaçâo em Saúde, Sociedade e Endemias na Amazônia. Manaus, AM, Brazil/ Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brazil/Universidade Federal do Pará. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Referência Nacional e Internacional para Flebotomíneos. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Referência Nacional e Internacional para Flebotomíneos. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Referência Nacional e Internacional para Flebotomíneos. Belo Horizonte, MG, Brazil.
Universidade Federal do Amazonas. Programa de Pós-Graduaçâo em Saúde, Sociedade e Endemias na Amazônia. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brazil/Universidade Federal do Pará. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Referência Nacional e Internacional para Flebotomíneos. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
Abstract: Phlebotominae sand flies are of medical importance because they are vectors of human pathogens, such as protozoa of the genus Leishmania Ross, etiological agent of American cutaneous leishmaniasis (ACL). In Lábrea, a municipality in the state of Amazonas, Brazil, ACL is primarily associated with subsistence activities, such as collection and extraction of forest products, undertaken by both indigenous and nonindigenous people. Data on ACL in indigenous populations are scarce, such that there is little information on the identity of the etiologie agent(s), reservoir host(s) and insect vector(s). The aim of this work was to study the sand fly fauna collected during an 8-d surveillance of different habitats in the Indigenous Reserve Caititu, Lábrea. In total, 1,267 sand flies were collected in different habitats for eight consecutive days, of which 819 (64.6%) were females and 448 (35.4%) males, from 10 genera and 32 species. The most abundant genera were Psychodopygus (34.3%), Trichophoromyia (22.9%), and Nyssomyia (15.3%). The most abundant species were Trichophoromyia ubiquitalis (Mangabeira) (n = 235, 18.5%), Psychodopygus davisi(Root) (n = 228, 18.0%) and Nyssomyia antunesi (Coutinho) (n = 135, 10.7%). Direct sequencing of polymerase chain reaction products demonstrated the presence of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis in the following species of sand flies:Evandromyia apurinan (Shimabukuro, Silveira, & Silva), Nyssomyia umbratilis (Ward & Fraiha),Nyssomyia yuilli yuilli (Young & Porter), Ps. davisi, Sciopemyia servulolimai (Damasceno & Causey), and Th. ubiquitalis. The presence of natural infection by Leishmania detected in the sand fly species investigated in this study suggests their possible role in the transmission cycle of ACL in the studied area.
Keywords: Amazon
cutaneous leishmaniasis
indigenous people
sand fly
Issue Date: 2014
Publisher: Entomological Society of America
Citation: SILVA, Túlio Romão Ribeiro da et al. Molecular Detection of Leishmania in Sand Flies (Diptera: Psychodidae: Phlebotominae) Collected in the Caititu Indigenous Reserve of the Municipality of Lábrea, State of Amazonas, Brazil. Journal of Medical Entomology, Vol. 51, n. 6, p. 1276-1282, 2014
DOI: 10.1603/ME14025
ISSN: 0022-2585
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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