Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10488
Title: Molecular mechanism driving retroperitoneal adipocyte hypertrophy and hyperplasia in response to a high-sugar diet
Authors: Queiroz, Karina Barbosa de
Coimbra, Roney Santos
Ferreira, Amanda Rios
Carneiro, Claudia Martins
Paiva, Nivia Carolina Nogueira
Costa, Daniela Caldeira
Evangelista, Elisio Alberto
Sa, Renata Guerra
Affilliation: Universidade Federal de Ouro Preto. Nucleo de Pesquisas em Ciencias Biologicas. Laboratorio de Bioquımica e Biologia Molecular. Ouro Preto, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Informatica de Biossistemas. Belo Horizonte, MG, Brasil
Universidade Federal de Ouro Preto. Nucleo de Pesquisas em Ciencias Biologicas. Laboratorio de Bioquımica e Biologia Molecular. Ouro Preto, MG, Brasil
Universidade Federal de Ouro Preto. Nucleo de Pesquisas em Ciencias Biologicas Laboratorio de Imunopatologia. Ouro Preto, MG, Brasil
Universidade Federal de Ouro Preto. Nucleo de Pesquisas em Ciencias Biologicas Laboratorio de Imunopatologia. Ouro Preto, MG, Brasil
Universidade Federal de Ouro Preto. Nucleo de Pesquisas em Ciencias Biologicas. Laboratorio de Bioquımica e Biologia Molecular. Ouro Preto, MG, Brasil
Universidade Federal de Ouro Preto. Nucleo de Pesquisas em Ciencias Biologicas. Laboratorio de Bioquımica e Biologia Molecular. Ouro Preto, MG, Brasil
Universidade Federal de Ouro Preto. Nucleo de Pesquisas em Ciencias Biologicas. Laboratorio de Bioquımica e Biologia Molecular. Ouro Preto, MG, Brasil
Abstract: Scope: We have previously shown an increase in adipocyte size and lipid content in retroperitoneal white adipose tissue (rWAT) induced by an 8-week high-sugar diet (HSD). In this study, we assessed the effect of a HSD on the transcriptional activity of adipogenic genes in a timecourse study to provide insight regarding the genetic networks involved in the rWAT response to dietary sugar. Methods and results: Weaned male Wistar rats were fed a standard chow diet or HSD (68% carbohydrates) for 4, 8 or 12 weeks, and rWAT was removed for histopathology and PCR array (adipogenesis) analyses. The HSD induced adipocyte hypertrophy and hyperplasia in rWAT after 12 weeks of ingestion. Additionally, the HSD altered serum VLDL-cholesterol, triacylglycerol and glucometabolic parameters. Hierarchical clustering revealed HSD-induced changes in the expression patterns of the tested gene set. Pathway analysis, which used the enrichment analysis algorithm of the Thompson Reuters MetaCore platform, associated a cluster of differentially expressed genes with canonical pathways related to regulating adipocyte differentiation and proliferation (p-value < 10−7). Conclusion: HSD feeding post-weaning increased both the adipocyte size and number by simultaneously up-regulating pro-adipogenic signals (the PPAR_ pathway) and down-regulating anti-adipogenic signals (Wnt pathway) in young adults
Keywords: High-sugar diet
Insulin resistance
PCR array
Systems biology
Unsupervised machine learning
Issue Date: 2014
Publisher: Weinheim
Citation: QUEIROZ, Karina Barbosa de et al. Molecular mechanism driving retroperitoneal adipocyte hypertrophy and hyperplasia in response to a high-sugar diet. Mol Nutr Food Res., v. 58, n. 12, p. 2331-41, 2014
DOI: 10.1002/mnfr.201400241
ISSN: 1613-4125
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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