| Author | Cruz, Aline | |
| Author | Mendes, Erica Araujo | |
| Author | Andrade, Marcus Vinicius Melo de | |
| Author | Nascimento, Vanuza Chagas do | |
| Author | Cartelle, Christiane Teixeira | |
| Author | Arantes, Rosa Maria Esteves | |
| Author | Melo, Jose Renan da Cunha | |
| Author | Gazzinelli, Ricardo Tostes | |
| Author | Ropert, Catherine | |
| Access date | 2015-05-25T15:37:38Z | |
| Available date | 2015-05-25T15:37:38Z | |
| Document date | 2014 | |
| Citation | CRUZ, Aline et al. Mast cells are crucial in the resistance against Toxoplasma gondii oral infection. Eur. J. Immunol, vol. 44, p. 2949–2954, 2014. | pt_BR |
| ISSN | 1521-4141 | |
| URI | https://www.arca.fiocruz.br/handle/icict/10491 | |
| Language | eng | pt_BR |
| Publisher | Wiley Online Library | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Mast cells are crucial in the resistance against Toxoplasma gondii oral infection | pt_BR |
| Type | Article | |
| DOI | 10.1002/eji.201344185 | |
| Abstract | During oral infection, mucosal immunity assumes a predominant role. Here, we addressed the role of mast cells (MCs), which are mainly located in mucosa during oral infection with Toxoplasma gondii, using MC-deficient (W/Wv) mice. We show that in the absence of MCs the resistance of W/Wv mice to oral infection was considerably reduced. W/Wv mice uniformly succumbedwithin 15 days of infection after administration of cysts of the ME49 strain of T. gondii. The rapid lethality of T. gondii in W/Wv mice correlated with a delayed Th1-cell response, since IFN-γ and IL-12 levels peaked in the later phase of the infection. In vitro, BM-derived MCs were able to recognize parasite lysate in a MyD88-dependent way, reaffirming the role of this TLR adapter in immune responses to T. gondii. The importance of MCs in vivo was confirmed when W/Wv mice reconstituted with BM-derived MCs from control mice retrieved an early strong Th1-cell response and specially a significant IL-12 production. In conclusion, MCs play an important role for the development of a protective immune response during oral infection with T. gondii. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Medicina. Belo Horizonte, MG, Brazil/ Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquimica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Imunopatologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Medicina. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Medicina. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Medicina. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Imunopatologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquimica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquimica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Mast cells | pt_BR |
| Subject | Mucosal immunity | pt_BR |
| Subject | Oral infection | pt_BR |
| Subject | Th1-cell response | pt_BR |
| Subject | Toxoplasma Gondii | pt_BR |
