Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10491
Title: Mast cells are crucial in the resistance against Toxoplasma gondii oral infection
Authors: Cruz, Aline
Mendes, Erica Araujo
Andrade, Marcus Vinicius Melo de
Nascimento, Vanuza Chagas do
Cartelle, Christiane Teixeira
Arantes, Rosa Maria Esteves
Melo, Jose Renan da Cunha
Gazzinelli, Ricardo Tostes
Ropert, Catherine
Affilliation: Universidade Federal de Minas Gerais. Escola de Medicina. Belo Horizonte, MG, Brazil/ Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquimica e Imunologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Imunopatologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Escola de Medicina. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Escola de Medicina. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Escola de Medicina. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Imunopatologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquimica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquimica e Imunologia. Belo Horizonte, MG, Brazil
Abstract: During oral infection, mucosal immunity assumes a predominant role. Here, we addressed the role of mast cells (MCs), which are mainly located in mucosa during oral infection with Toxoplasma gondii, using MC-deficient (W/Wv) mice. We show that in the absence of MCs the resistance of W/Wv mice to oral infection was considerably reduced. W/Wv mice uniformly succumbedwithin 15 days of infection after administration of cysts of the ME49 strain of T. gondii. The rapid lethality of T. gondii in W/Wv mice correlated with a delayed Th1-cell response, since IFN-γ and IL-12 levels peaked in the later phase of the infection. In vitro, BM-derived MCs were able to recognize parasite lysate in a MyD88-dependent way, reaffirming the role of this TLR adapter in immune responses to T. gondii. The importance of MCs in vivo was confirmed when W/Wv mice reconstituted with BM-derived MCs from control mice retrieved an early strong Th1-cell response and specially a significant IL-12 production. In conclusion, MCs play an important role for the development of a protective immune response during oral infection with T. gondii.
Keywords: Mast cells
Mucosal immunity
Oral infection
Th1-cell response
Toxoplasma Gondii
Issue Date: 2014
Publisher: Wiley Online Library
Citation: CRUZ, Aline et al. Mast cells are crucial in the resistance against Toxoplasma gondii oral infection. Eur. J. Immunol, vol. 44, p. 2949–2954, 2014.
DOI: 10.1002/eji.201344185
ISSN: 1521-4141
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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