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https://www.arca.fiocruz.br/handle/icict/10570
EFFECTIVENESS OF ANTIMICROBIAL PHOTODYNAMIC THERAPY ON STAPHYLOCOCCUS AUREUS USING PHENOTHIAZINIUM DYE WITH RED LASER
Author
Affilliation
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Estate University of Feira de Santana. Department of Biology. Feira de Santana, BA, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil / Social Changes. Asthma and Allergy in Latin America. SCAALA. Salvador, BA, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil / Social Changes. Asthma and Allergy in Latin America. SCAALA. Salvador, BA, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil
Abstract
The aim of this study was to evaluate in vitro the bactericidal effect of Antimicrobial Photodynamic Therapy - AmPDT
using a phenothiazinium compound (toluidine blue O and methylene blue, 12.5 μg/mL) on Staphylococcus aureus
(ATCC 23529) irradiated or not with the red laser (λ 660 nm, 12J/cm2). All tests were performed in triplicate and
samples distributed into the following groups: Negative control, Laser, Photosensitizer, and AmPDT. Bactericidal effect
of the Antimicrobial Photodynamic Therapy was assessed by counting of colony-forming units and analyzed statistically
(ANOVA, Tukey test, p<0.05). The results showed, comparing the Laser group with Negative control, a statistically
significant increase of counting on the Laser group (p = 0.003). The use of the photosensitizer alone reduced the mean
number of CFU (64.8%) and its association with the Laser light resulted in 84.2% of inhibition. The results are indicative
that the use of Antimicrobial Photodynamic Therapy presented in vitro bactericidal effect on Staphylococcus aureus.
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