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EFFECTIVENESS OF ANTIMICROBIAL PHOTODYNAMIC THERAPY ON STAPHYLOCOCCUS AUREUS USING PHENOTHIAZINIUM DYE WITH RED LASER
Monteiro, Juliana Santos de Carvalho et al. | Date Issued: 2014
Author
Monteiro, Juliana Santos de Carvalho
Oliveira, Susana Carla Pires Sampaio
Santos, Gustavo Miranda Pires
Sampaio, Fernando José Pires
Zanin, Fátima Antônia Aparecida
Pinheiro, Antônio Luiz Barbosa
Affilliation
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Estate University of Feira de Santana. Department of Biology. Feira de Santana, BA, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil / Social Changes. Asthma and Allergy in Latin America. SCAALA. Salvador, BA, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil
Abstract
The aim of this study was to evaluate in vitro the bactericidal effect of Antimicrobial Photodynamic Therapy - AmPDT using a phenothiazinium compound (toluidine blue O and methylene blue, 12.5 μg/mL) on Staphylococcus aureus (ATCC 23529) irradiated or not with the red laser (λ 660 nm, 12J/cm2). All tests were performed in triplicate and samples distributed into the following groups: Negative control, Laser, Photosensitizer, and AmPDT. Bactericidal effect of the Antimicrobial Photodynamic Therapy was assessed by counting of colony-forming units and analyzed statistically (ANOVA, Tukey test, p<0.05). The results showed, comparing the Laser group with Negative control, a statistically significant increase of counting on the Laser group (p = 0.003). The use of the photosensitizer alone reduced the mean number of CFU (64.8%) and its association with the Laser light resulted in 84.2% of inhibition. The results are indicative that the use of Antimicrobial Photodynamic Therapy presented in vitro bactericidal effect on Staphylococcus aureus.
Keywords
Photodynamic antimicrobial chemotherapy
Staphylococcus aureus
Lasers
 
Publisher
Society of Photo-optical Instrumentation Engineers (SPIE)
Citation
MONTEIRO, J. S. C. et al. Effectiveness of antimicrobial photodynamic therapy on staphylococcus aureus using phenothiazinium dye with red laser. Proceedings of SPIE, v. 9309, p. 93090X-1, 2014.
DOI
10.1117/12.2079170
ISSN
0277-786X