Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10611
Title: Characterization of carbapenem-resistant Acinetobacter baumannii in Brazil (2008–2011): countrywide spread of OXA-23–producing clones (CC15 and CC79)
Authors: Chagas, Thiago Pavoni Gomes
Carvalho, Karyne Rangel
Santos, Ivson Cassiano de Oliveira
Assef, Ana Paula D`Alincourt Carvalho
Asensi, Marise Dutra
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.
Abstract: The study investigated the genetic relationship of carbapenem-resistant Acinetobacter baumannii clinical isolated from inpatients during 2008–2011 from 11 Brazilian states. Antimicrobial susceptibility profile was determined by disc diffusion method and Etest. Polymerase chain reaction was applied for carbapenemase genes, and ISAba1. Isolates were subjected to pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) for molecular typing. Most of the isolates showed high resistance rates to antibiotics tested. The blaOXA-51-like gene was found in all isolates, and 146 (94.2%) isolates were positive for blaOXA-23-like. In the most OXA-23–producing isolates, the blaOXA-23-like gene was accompanied by ISAba1. A total of 146 OXA-23–producing isolates were clustered into 28 genotypes by PFGE. Molecular analysis by MLST identified 13 sequence types (STs). The most prevalent PFGE profiles were designated as ST15 (CC15), ST1 (CC1), and ST79 (CC79). This study showed the widespread of clonal complexes of A. baumannii harboring the blaOXA-23-like gene in different Brazilian states.
Keywords: Acinetobacter baumannii
Brazil
Carbapenem resistance
OXA-23
MLST
DeCS: Acinetobacter baumannii
Brasil
Issue Date: 2014
Publisher: Elsevier
Citation: CHAGAS, Thiago Pavoni Gomes et al. Characterization of carbapenem-resistant Acinetobacter baumannii in Brazil (2008–2011): countrywide spread of OXA-23–producing clones (CC15 and CC79). Diagnostic Microbiology and Infectious Disease, v. 79, n.4, p.468–472, 2014.
DOI: 10.1016/j.diagmicrobio.2014.03.006
ISSN: 0732-8893
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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