Author | Araujo, Leonardo Silva de | |
Author | Mello, Fernanda Carvalho de Queiroz | |
Author | Silva, Nidai de Bárbara Moreira da | |
Author | Leung, Janaina Aparecida Medeiros | |
Author | Machado, Silvia Maria Almeida | |
Author | Sardella, Isabela Gama | |
Author | Maciel, Renata de Moraes | |
Author | Saad, Maria Helena Féres | |
Access date | 2015-06-01T19:34:28Z | |
Available date | 2015-06-01T19:34:28Z | |
Document date | 2014 | |
Citation | ARAUJO, Leonardo Silva de et al. Evaluation of Gamma Interferon Immune Response Elicited by the Newly Constructed PstS-1(285-374):CFP10 Fusion Protein To Detect Mycobacterium tuberculosis Infection. Clin Vaccine Immunol., v.21, n.4, p.552–560. apr. 2014. | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/10636 | |
Language | eng | pt_BR |
Publisher | PubMed Central | pt_BR |
Rights | open access | |
Subject in Portuguese | Tuberculose | pt_BR |
Title | Evaluation of Gamma Interferon Immune Response Elicited by the Newly Constructed PstS-1(285-374):CFP10 Fusion Protein To Detect Mycobacterium tuberculosis Infection | pt_BR |
Type | Article | |
DOI | 10.1128/CVI.00726-13 | pt_BR |
Abstract | The PstS1 antigen is highly immunogenic, principally when combined with CFP10 during both latent and active TB infection. In
the present study, a selected pstS1 gene fragment was cloned, fused with CFP10, and expressed in Escherichia coli. The product
[PstS-1(285-374):CFP10] was compared to the recombinant fused RD1 (region of deletion 1) protein (ESAT-6:CFP10) in detecting
Mycobacterium tuberculosis infection in 108 recent contacts of pulmonary tuberculosis (TB) cases, considering a positive
tuberculin skin test (TST) to be the baseline. The release of gamma interferon (IFN- ) in 22-h whole-blood and 5-day lymphocyte
stimulation assays primed with each antigen was determined. All contacts were clinically followed for up to 1 year, and 87%
of the tuberculin skin test-positive (TSTpositive) patients accepted preventative treatment. Concerning the IFN- response to
PstS-1(285-374):CFP10 in the 22-h and 5-day assays, a slight increase in contact-TSTpositive detection was observed (23/54 and
26/54) compared to the level seen with the RD1 protein (18/54 and 24/54) whereas in the TSTnegative group, similarly lower numbers
(<5/48) of responders were achieved for both antigens, except for RD1 in the 5-day assay (8/48). By combining the IFN-
responders to both antigens in the 5-day assays, slightly higher increases in positivity were found in the TSTpositive (32/54) and
TSTnegative (10/48) groups. Two of 12 untreated TSTpositive contacts progressed to active TB and were concordantly positive in all
assays, except for one contact who lacked positivity in the RD1 5-day assay. We demonstrated for the first time that PstS-1(285-
374):CFP10 slightly increased contact positivity and detection of active disease progression, suggesting its potential application
as a TB infection marker. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Mycobacterium tuberculosis | pt_BR |
DeCS | Mycobacterium tuberculosis | pt_BR |