Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10671
Title: 1,10-Phenanthroline Inhibits the Metallopeptidase Secreted by Phialophora verrucosa and Modulates its Growth, Morphology and Differentiation
Authors: Granato, Marcela Queiroz
Massapust, Priscila de Araújo
Rozenthal, Sonia
Alviano, Celuta Sales
Santos, André Luis Souza dos
Kneipp, Lucimar Ferreira
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biologia Celular de Fungos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Superfície de Microrganismos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases. Programa de Pós-Graduação em Bioquímica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.
Abstract: Phialophora verrucosa is one of the etiologic agents of chromoblastomycosis, a fungal infection that affects cutaneous and subcutaneous tissues. This disease is chronic, recurrent and difficult to treat. Several studies have shown that secreted peptidases by fungi are associated with important pathophysiological processes. Herein, we have identified and partially characterized the peptidase activity secreted by P. verrucosa conidial cells. Using human serum albumin as substrate, the best hydrolysis profile was detected at extreme acidic pH (3.0) and at 37 °C. The enzymatic activity was completely blocked by classical metallopeptidase inhibitors/chelating agents as 1,10-phenanthroline and EGTA. Zinc ions stimulated the metallo-type peptidase activity in a dose-dependent manner. Several proteinaceous substrates were cleaved, in different extension, by the P. verrucosa metallopeptidase activity, including immunoglobulin G, fibrinogen, collagen types I and IV, fibronectin, laminin and keratin; however, mucin and hemoglobin were not susceptible to proteolysis. As metallopeptidases participate in different cellular metabolic pathways in fungal cells, we also tested the influence of 1,10-phenanthroline and EGTA on P. verrucosa development. Contrarily to EGTA, 1,10-phenanthroline inhibited the fungal viability (MIC 0.8 µg/ml), showing fungistatic effect, and induced profound morphological alterations as visualized by transmission electron microscopy. In addition, 1,10-phenanthroline arrested the filamentation process in P. verrucosa. Our results corroborate the supposition that metallopeptidase inhibitors/chelating agents have potential to control crucial biological events in fungal agents of chromoblastomycosis.
Keywords: Phialophora verrucosa
Chromoblastomycosis
Metallopeptidase
1,10-Phenanthroline
Growth
Differentiation
keywords: Diferenciação
Crescimento
Dermatitis Verrucosa
DeCS: Cromoblastomicose
Issue Date: 2014
Publisher: SpringerLink
Citation: GRANATO, Marcela Queiroz et al. 1,10-Phenanthroline Inhibits the Metallopeptidase Secreted by Phialophora verrucosa and Modulates its Growth, Morphology and Differentiation. Mycopathologia, v.179, n.3-4, ´.231-242, 2014.
DOI: 10.1007/s11046-014-9832-7
ISSN: 1573-0832
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
marcela_granatoetal_IOC_2014.pdf770.55 kBAdobe PDFView/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.