Title:	Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
Authors:	Vieira, Cecilia Stahl Almeida, Diogo Burigo Thomaz, André Alexandre de Menna-Barreto, Rubem Figueredo Sadok Santos-Mallet, Jacenir R. Cesar, Carlos Lenz Gomes, Suzete Araujo Oliveira Feder, Denise
Affilliation:	Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil. Universidade Estadual de Campinas.Instituto de Física Gleb Wataghin. Laboratório de Aplicações Biomédicas de Lasers. Campinas, SP, Brasil. Universidade Estadual de Campinas.Instituto de Física Gleb Wataghin. Laboratório de Aplicações Biomédicas de Lasers. Campinas, SP, Brasil Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil. Universidade Estadual de Campinas.Instituto de Física Gleb Wataghin. Laboratório de Aplicações Biomédicas de Lasers. Campinas, SP, Brasil. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil
Abstract:	Semiconductor nanoparticles, such as quantum dots (QDs), were used to carry out experiments in vivo and ex vivo with Trypanosoma cruzi. However, questions have been raised regarding the nanotoxicity of QDs in living cells, microorganisms, tissues and whole animals. The objective of this paper was to conduct a QD nanotoxicity study on living T. cruzi protozoa using analytical methods. This was accomplished using in vitro experiments to test the interference of the QDs on parasite development, morphology and viability. Our results show that after 72 h, a 200 μM cadmium telluride (CdTe) QD solution induced important morphological alterations in T. cruzi, such as DNA damage, plasma membrane blebbing and mitochondrial swelling. Flow cytometry assays showed no damage to the plasma membrane when incubated with 200 μM CdTe QDs for up to 72 h (propidium iodide cells), giving no evidence of classical necrosis. Parasites incubated with 2 μM CdTe QDs still proliferated after seven days. In summary, a low concentration of CdTe QDs (2 μM) is optimal for bioimaging, whereas a high concentration (200 μM CdTe) could be toxic to cells. Taken together, our data indicate that 2 μM QD can be used for the successful long-term study of the parasite-vector interaction in real time.
Keywords:	CdTe quantum dots Trypanosoma cruzi Nanotoxicity
DeCS:	Trypanosoma cruzi
Issue Date:	2011
Publisher:	Fundação Oswaldo Cruz
Citation:	VIEIRA, Cecilia Stahl et al. Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi. Mem Inst Oswaldo Cruz, v.106, n.2, p.158-165, Mar. 2011.
ISSN:	1678-8060
Copyright:	open access
Appears in Collections:	IOC - Artigos de Periódicos

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