| Autor | Bonin, Renata Fajardo | |
| Autor | Chapeaurouge, Alex | |
| Autor | Perales, Jonas | |
| Autor | Silva Jr., José Godinho da | |
| Autor | Nascimento, Hilton Jorge do | |
| Autor | Assef, Ana Paula D`Alincourt Carvalho | |
| Autor | Senna, José Procópio Moreno | |
| Data de acesso | 2015-06-08T14:01:53Z | |
| Data de disponibilização | 2015-06-08T14:01:53Z | |
| Data do publicação | 2014 | |
| Citação | BONIN, Renata Fajardo et al. Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach. Proteomics Clin. Appl., v.00, p.1-8, 2014. | pt_BR |
| ISSN | 1862-8354 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10704 | |
| Idioma | eng | pt_BR |
| Editor | WILEY-VCH Verlag GmbH & Co | pt_BR |
| Direito Autoral | open access | |
| Palavras-chave | Infecção hospitalar | pt_BR |
| Título | Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach | pt_BR |
| Tipo do documento | Article | |
| DOI | 10.1002/prca.201300133 | pt_BR |
| Resumo em Inglês | Purpose: Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae,
urinary tract infections, and/or septicemia in immunocompromised patients. This
pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly
problematic because of its prevalence and resistance patterns to several antibiotics. In
the present study, we used an immunoproteome-based approach to identify immunogenic proteins
located on the surface of A. baumannii for the development of a possible immunotherapy
against this devastating bacterial infection.
Experimental design: Sera from patients with A. baumannii infections (n = 50) and from a
control group of healthy individuals (n = 3) were analyzed for reactivity against A. baumannii
outer membrane proteins (OMPs) using Western blot analysis. To identify potential immunogenic proteins in A. baumannii,OMPs were separated by 2DE (2Delectrophoresis), and reactive
sera from infected patients were randomly selected and divided into two different pools, each
containing 15 sera. Finally, MALDI-TOF/TOF mass spectrometric analysis was employed to
identify the corresponding proteins.
Results: This analysis identified six immunoreactive proteins: OmpA, Omp34kDa, OprC,
OprB-like, OXA-23, and ferric siderophore receptor protein. Notably, these proteins are highly
abundant on the bacterial surface and involved in virulence, antibiotic resistance, and growth.
Conclusions and clinical relevance: Our results support the notion that the proteins identified
in the present immunoproteome study could serve as antigen candidates for the development
of vaccines and passive immunotherapies against A. baumannii infections. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliação | undação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliação | undação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa de Infecções Hospitalares. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Palavras-chave em inglês | Acinetobacter baumannii | pt_BR |
| Palavras-chave em inglês | Hospital infection | pt_BR |
| Palavras-chave em inglês | Immunoproteomics | pt_BR |
| Palavras-chave em inglês | MALDI-TOF/TOF | pt_BR |
| DeCS | Acinetobacter baumannii | pt_BR |
| DeCS | Proteômica | pt_BR |
