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SENSITIVITY AND SPECIFICITY OF SEROLOGICAL TESTS, HISTOPATHOLOGYAND IMMUNOHISTOCHEMISTRY FOR DETECTION OF TOXOPLASMAGONDII INFECTION IN DOMESTIC CHICKENSL
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tSince free-range chickens are important for the epidemiology of toxoplasmosis, this studyevaluated the sensitivity and specificity of different laboratory techniques for the diag-nosis of Toxoplasma gondii in these animals. Serum samples from 135 adult domesticchickens were tested for anti-T. gondii antibodies by the indirect fluorescent antibody test(IFAT), enzyme-linked immunosorbent assay (ELISA), modified agglutination test (MAT),and indirect hemagglutination test (IHAT). Tissue samples from all animals were analyzedby histopathology, immunohistochemistry and mouse bioassay (gold standard). Fifty-fourchickens were positive for T. gondii in the bioassay. The sensitivity and specificity of thedifferent tests were, respectively, 85% and 56% for ELISA; 80% and 52% for IFAT; 76% and68% for MAT; 61% and 80% for IHAT; 7% and 98% for immunohistochemistry, and 6% and 98%for histopathology. The MAT was the most effective method for the diagnosis of T. gondiiinfection in chickens, followed by ELISA. Histopathology and immunohistochemistry areuseful tools for the diagnosis of T. gondii infection in chickens due to their specificity.
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