Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10826
Title: A decade of G3P[8] and G9P[8] rotaviruses in Brazil: Epidemiology and evolutionary analyses
Authors: Gómez, Mariela Martínez
Costa, Filipe Anibal Carvalho
Volotão, Eduardo de Mello
Rose, Tatiana Lundgren
Silva, Marcelle Fiqueira Marques da
Fialho, Alexandre Madi
Assis, Rosane Maria Santos de
Matthijnssens, Jelle
Leite, José Paulo Gagliardi
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
University of Leuven. Rega Institute for Medical Research. Department of Microbiology and Immunology. Laboratory of Clinical and Epidemiological. Leuven, Belgium.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Abstract: This study aims to estimate the frequency of group A rotaviruses (RVA) infection with genotypes G3P[8] and G9P[8] in children that suffered from diarrheal disease (DD) between 2001 and 2011 in different Brazilian regions. In addition, the genetic diversity of G3P[8] and G9P[8] RVA strains recovered from vaccinated and non-vaccinated children was assessed. Laboratory-based RVA surveillance included 15,115 cases of DD, and RVA was detected by enzyme immune-assay and/or polyacrylamide gel electrophoresis in 3357 (22%) samples. RVA was genotyped by the semi-nested RT-PCR and among RVA-positive samples, 100 (2.9%) were G3 (63 G3P[8], 32 G3P not typed [NT], and 5 G3P[6]) and 378 (16.2%) were G9 (318 G9P[8], 59 G9P[NT], and 1 G9P[6]). From the G3 and G9 positive samples, 16 and 12, respectively, were obtained from children aged 4–48 months vaccinated with the monovalent vaccine (Rotarix , RV1). Phylogenetic analyses of the VP7 and VP8⁄ encoding genes were performed for 26 G3P[8] and 48 G9P[8] strains. VP8⁄ phylogenetic analysis revealed that all strains analyzed belonged to P[8] lineage III, whereas RV1 belongs to P[8]-I lineage. VP7 analysis revealed that all G3 and G9 strains belonged to G3-lineage III and G9-lineage III. The comparison of the VP7 and VP8⁄ antigenic epitopes regions of Brazilian strains with RV1 strain revealed several amino acid changes. However, no particular differences among Brazilian strains detected before and after vaccine introduction were observed, or among strains detected from vaccinated and non-vaccinated children. Complete genome characterization of four G3P[8] and seven G9P[8] strains revealed a typical conserved human Wa-like genomic constellation. Changes in the genetic diversity of G3P[8] and G9P[8] RVA detected from 2001 to 2011 in Brazil seemed not be related to RV1 introduction in Brazil.
Keywords: Diarrheal disease
Group A rotaviruses
Genotypes G3 and G9
Phylogenetic analysis
Monovalent vaccine
DeCS: Infecções por Rotavirus
Rotavirus
Genótipo
Diarreia
Vacinas
Issue Date: 2014
Publisher: Elsevier
Citation: GÓMEZ, Mariela Martínez et al. A decade of G3P[8] and G9P[8] rotaviruses in Brazil: Epidemiology and evolutionary analyses. Infection, Genetics and Evolution, v.28, p.389–397, 2014.
DOI: 10.1016/j.meegid.2014.05.016
ISSN: 1567-1348
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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