Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10872
Title: Detection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazil
Authors: Menezes, Francisca Gleire Rodrigues de
Neves, Soraya da Silva
Sousa, Oscarina Viana de
Vila-Nova, Candida Machado Veira Maia
Maggioni, Rodrigo
Theophilo, Grace Nazareth Diogo
Hofer, Ernesto
Vieira, Regine Helena Silva dos Fernandes
Affilliation: Universidade Federal do Ceará. Departamento de Engenharia da Pesca. Fortaleza, CE, Brasil.
Universidade Federal do Ceará. Departamento de Engenharia da Pesca. Fortaleza, CE, Brasil.
Universidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.
Universidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.
Universidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.
Abstract: The objectives of this study were to detect the presence of Vibrio cholerae in tropical estuaries (Northeastern Brazil) and to search for virulence factors in the environmental isolates. Water and sediment samples were inoculated onto a vibrio-selective medium (TCBS), and colonies with morphological resemblance to V. cholerae were isolated. The cultures were identified phenotypically using a dichotomous key based on biochemical characteristics. The total DNA extracted was amplified by PCR to detect ompW and by multiplex PCR to detect the virulence genes ctx, tcp, zot and rfbO1. The results of the phenotypic and genotypic identification were compared. Nine strains of V. cholerae were identified phenotypically, five of which were confirmed by detection of the species-specific gene ompW. The dichotomous key was efficient at differentiating environmental strains of V. cholerae. Strains of V. cholerae were found in all four estuaries, but none possessed virulence genes.
Keywords: Cholera
Estuaries
Pathogenicity
Genes
DeCS: Cólera
Virulência
Genes
Estuários
Issue Date: 2014
Publisher: Instituto de Medicina Tropical de São Paulo
Citation: MENEZES, Francisca Gleire Rodrigues de; et al. Detection of virulence genes in environmental strains of Vibrio cholerae from Estuaries in Northeastern Brasil.Rev Inst Med Trop Sao Paulo. v.56, n.5, p.427-32, Sept. Oct. 2014.
DOI: 10.1590/S0036-46652014000500010
ISSN: 0036-4665
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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