Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10876
Title: Polymorphisms at Residue 222 of the Hemagglutinin of Pandemic Influenza A(H1N1)pdm09: Association of Quasi-Species to Morbidity and Mortality in Different Risk Categories
Authors: Resende, Paola Cristina
Motta, Fernando C
Olliveira, Maria de Lourdes A
Gregianini, Tatiana S
Fernandes, Sandra B
Cury, Ana Luisa F
Rosa, Maria do Carmo D
Souza, Thiago Moreno L
Siqueira, Marilda M
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ, Brasil.
Fundação Estadual de Produção e Pesquisa em Saúde. Seção de Virologia. Laboratório Central de Saúde Pública do Estado do Rio de Grande do Sul. Porto Alegre, RS. Brasil.
Laboratório Central de Saúde Pública do Estado de Santa Catarina. Florianópolis, SC, Brasil.
Laboratório Central de Saúde Pública do Estado de Minas Gerais / Instituto Octávio Magalhães / Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil.
Laboratório Central de Saúde Pública do Estado do Paraná. Curitiba, PR, Brasil.
Abstract: The D222G substitution in the hemagglutinin (HA) gene of the pandemic influenza A(H1N1)pdm09 virus has been identified as a potential virulence marker, because this change allows for virus invasion deeper into the respiratory tract. In this study, we analyzed D, G and N polymorphisms at residue 222 by pyrosequencing (PSQ). We initially analyzed 401 samples from Brazilian patients. These were categorized with respect to clinical conditions due to influenza infection (mild, serious or fatal) and sub-stratified by risky factors. The frequency of mixed population of virus, with more than one polymorphism at residue 222, was significantly higher in serious (10.6%) and fatal (46.7%) influenza cases, whereas those who showed mild influenza infections were all infected by D222 wild-type. Mixtures of quasi-species showed a significant association of mortality, especially for those with risk factors, in special pregnant women. These results not only reinforce the association between D222G substitution and influenza A(H1N1)pdm09-associated morbidity and mortality, but also add the perspective that a worse clinical prognosis is most likely correlated with mixtures of quasi-species at this HA residue. Therefore, quasi-species may have a critical and underestimated role in influenza-related clinical outcomes.
Keywords: Pandemic Influenza A(H1N1)pdm09
Polymorphisms
Residue 222
Hemagglutinin
DeCS: Doenças Respiratórias
Issue Date: 2014
Publisher: Plos One
Citation: RESENDE, Paola Cristina et al. Polymorphisms at Residue 222 of the Hemagglutinin of Pandemic Influenza A(H1N1)pdm09: Association of Quasi-Species to Morbidity and Mortality in Different Risk Categories. Plos One, v.9, n.3, 5p, mar. 2014
DOI: 10.1371/ journal.pone.0092789
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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