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NHUMIRIM VIRUS, A NOVEL FLAVIVIRUS ISOLATED FROM MOSQUITOES FROM THE PANTANAL, BRAZIL
Corrêa, Alex Pauvolid et al. | Date Issued: 2015
Author
Corrêa, Alex Pauvolid
Solberg, Owen
Lima, Dinair Couto
Kenney, Joan
Freire, Nicolau Serra
Brault, Aaron
Nogueira, Rita
Langevin, Stanley
Komar, Nicholas
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Sandia National Laboratories. Systems Biology Department. Livermore, CA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Centers for Disease Control and Prevention. Division of Vector-Borne Diseases. For Collins, CO, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Refereˆncia Nacional para Vetores das Riquetsioses. Rio de Janeiro, RJ, Brasil.
Centers for Disease Control and Prevention. Division of Vector-Borne Diseases. For Collins, CO, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Sandia National Laboratories. Systems Biology Department. Livermore, CA, USA.
Centers for Disease Control and Prevention. Division of Vector-Borne Diseases. For Collins, CO, USA.
Abstract
We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.
Keywords
Flavivirus
Nhumirim virus
Pantanal
Brazil
Novel flavivirus
 
DeCS
Flaviviridae
Pantanal
Flavivirus
 
Publisher
Springer
Citation
CORRÊA, Alex Pauvolid et al. Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil. Arch Virol., v.1060, n.1, p. 21–27, 2015.
DOI
10.1007/s00705-014-2219-8
ISSN
0304-8608