Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10886
Title: DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors
Authors: Justi, Silvia Andrade
Dale, Caroline
Galvão, Cleber
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Biologia. Laboratório de Biologia Evolutiva Teórica e Aplicada.Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.
Abstract: Background: DNA barcoding assumes that a biological entity is completely separated from its closest relatives by a barcoding gap, which means that intraspecific genetic distance (from COI sequences) should never be greater than interspecific distances. We investigated the applicability of this strategy in identifying species of the genus Triatoma from South America. Findings: We calculated intra and interspecific Kimura-2-parameter distances between species from the infestans, matogrossensis, sordida and rubrovaria subcomplexes. In every subcomplex examined we observed at least one intraspecific distance greater than interspecific distances. Conclusions: Although DNA barcoding is a straightforward approach, it was not applicable for identifying Southern American Triatoma species, which may have diverged recently. Thus, caution should be taken in identifying vector species using this approach, especially in groups where accurate identification of taxa is fundamentally linked to public health issues.
Keywords: Triatominae
Chagas disease
DNA barcoding
Molecular identification
keywords: Doença de Chagas
Triatominae
Issue Date: 2014
Publisher: BioMed Central
Citation: JUSTI, Silvia Andrade; DALE, Carolina; GALVÃO, Cleber. DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors. Parasites & Vectors, v.7:519, 4p, 2014.
DOI: 10.1186/s13071-014-0519-1
ISSN: 1756-3305
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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