| Author | Van Weyenbergh, Johan Jozef Rosa Maria | |
| Author | Silva, Maria da Purificação Pereira da | |
| Author | Bafica, Andre Luiz Barbosa | |
| Author | Cardoso, Silvia | |
| Author | Wietzerbin, Juana | |
| Author | Barral Netto, Manoel | |
| Access date | 2015-06-30T16:31:52Z | |
| Available date | 2015-06-30T16:31:52Z | |
| Document date | 2001 | |
| Citation | VAN WEYENBERGH, Johan Jozef Rosa Maria et al. IFN-beta and TGF-beta differentially regulate IL-12 activity in human peripheral blood mononuclear cells. Immunology Letters, v. 75, n. 2, p. 117-122, 2001. | pt_BR |
| ISSN | 0165-2478 | |
| xmlui.metadata.dc.identifier.other | S0165-2478(00)00303-5 | |
| URI | https://www.arca.fiocruz.br/handle/icict/11017 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Title | IFN-beta and TGF-beta differentially regulate IL-12 activity in human peripheral blood mononuclear cells | pt_BR |
| Type | Article | |
| DOI | 10.1016/S0165-2478(00)00303-5 | pt_BR |
| Abstract | Both IFN-b and TGF-b have demonstrated their ability to antagonize several of the stimulatory activities of IFN-g on human macrophages, thereby classifying them as Th2-like. Aiming at a further characterization of their role in Th1:Th2 development, we studied their possible interaction with IL-12, the key Th1 cytokine. We found that IFN-b by itself induced modest amounts of IFN-g, but was able to synergize with IL-12 for IFN-g induction. TGF-b, on the other hand, had no effect by itself and inhibited significantly the IL-12-induced IFN-g secretion. The differential effect of IFN-b and TGF-b on IL-12 bioactivity was most pronounced upon IFN-g synthesis, since IFN-b induced only marginal amounts of IL-10 and IL-12 and TGF-b diminished constitutive IL-10 production, while neither had a significant effect on TNF-a production. Although monocytes did not produce detectable IFN-g with any of the stimuli, adherent cells were found to cooperate with non-adherent lymphocytes for maximal IFN-g production. However, IL-18, a monocyte-derived IFN-g-inducing cytokine able to synergize with IL-12, was undetectable in IFN-b or IFN-b IL-12-stimulated cells. In conclusion, the ability of IFN-b to synergize with IL-12 for IFN-g synthesis, without significant concomitant IL-10 production, suggest a strong boost to Th1 development, which seems to be IL-18-independent. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Immunoregulação e Microbiologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Immunoregulação e Microbiologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Immunoregulação e Microbiologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Immunoregulação e Microbiologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | INSERM. Institut Curie. Section de Recherche. Paris, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Immunoregulação e Microbiologia. Salvador, BA, Brasil. | pt_BR |
| Subject | IFN-g | pt_BR |
| Subject | Synergy | pt_BR |
| Subject | Antagonism | pt_BR |
| Subject | Leishmaniasis | pt_BR |
| Subject | Multiple sclerosis | pt_BR |
| DeCS | Interferon Tipo I/farmacologia | pt_BR |
| DeCS | Interleucina-12/metabolismo | pt_BR |
| DeCS | Leucócitos Mononucleares/efeitos de drogas | pt_BR |
| DeCS | Leucócitos Mononucleares/imunologia | pt_BR |
| DeCS | Fator de Crescimento Transformador beta/farmacologia | pt_BR |
| DeCS | Adesão Celular | pt_BR |
| DeCS | Interações de Medicamentos | pt_BR |
| DeCS | Técnicas In Vitro | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Interferon Tipo I/administração & dosagem | pt_BR |
| DeCS | Interferon gama/biossíntese | pt_BR |
| DeCS | Interleucina-12/administração & dosagem | pt_BR |
| DeCS | Leucócitos Mononucleares/citologia | pt_BR |
| DeCS | Proteínas Recombinantes | pt_BR |
| DeCS | Células Th1/efeitos de drogas | pt_BR |
| DeCS | Fator de Crescimento Transformador beta/administração & dosagem | pt_BR |
| DeCS | Fator de Necrose Tumoral alfa/biossíntese | pt_BR |
