Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11017
Title: IFN-beta and TGF-beta differentially regulate IL-12 activity in human peripheral blood mononuclear cells.
Authors: Van Weyenbergh, Johan Jozef Rosa Maria
Silva, Maria da Purificação Pereira da
Bafica, Andre Luiz Barbosa
Cardoso, Silvia
Wietzerbin, Juana
Barral Netto, Manoel
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Immunoregulação e Microbiologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Immunoregulação e Microbiologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Immunoregulação e Microbiologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Immunoregulação e Microbiologia. Salvador, BA, Brasil
INSERM. Institut Curie. Section de Recherche. Paris, France
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Immunoregulação e Microbiologia. Salvador, BA, Brasil
Abstract: Both IFN-b and TGF-b have demonstrated their ability to antagonize several of the stimulatory activities of IFN-g on human macrophages, thereby classifying them as Th2-like. Aiming at a further characterization of their role in Th1:Th2 development, we studied their possible interaction with IL-12, the key Th1 cytokine. We found that IFN-b by itself induced modest amounts of IFN-g, but was able to synergize with IL-12 for IFN-g induction. TGF-b, on the other hand, had no effect by itself and inhibited significantly the IL-12-induced IFN-g secretion. The differential effect of IFN-b and TGF-b on IL-12 bioactivity was most pronounced upon IFN-g synthesis, since IFN-b induced only marginal amounts of IL-10 and IL-12 and TGF-b diminished constitutive IL-10 production, while neither had a significant effect on TNF-a production. Although monocytes did not produce detectable IFN-g with any of the stimuli, adherent cells were found to cooperate with non-adherent lymphocytes for maximal IFN-g production. However, IL-18, a monocyte-derived IFN-g-inducing cytokine able to synergize with IL-12, was undetectable in IFN-b or IFN-b IL-12-stimulated cells. In conclusion, the ability of IFN-b to synergize with IL-12 for IFN-g synthesis, without significant concomitant IL-10 production, suggest a strong boost to Th1 development, which seems to be IL-18-independent
Keywords: IFN-g
Synergy
Antagonism
Leishmaniasis
Multiple sclerosis
DeCS: Interferon Tipo I/farmacologia
Interleucina-12/metabolismo
Leucócitos Mononucleares/efeitos de drogas
Leucócitos Mononucleares/imunologia
Fator de Crescimento Transformador beta/farmacologia
Adesão Celular
Interações de Medicamentos
Técnicas In Vitro
Humanos
Interferon Tipo I/administração & dosagem
Interferon gama/biossíntese
Interleucina-12/administração & dosagem
Leucócitos Mononucleares/citologia
Proteínas Recombinantes
Células Th1/efeitos de drogas
Fator de Crescimento Transformador beta/administração & dosagem
Fator de Necrose Tumoral alfa/biossíntese
Issue Date: 2001
Publisher: Elsevier
Citation: VAN WEYENBERGH, J. J. R. M. et al. IFN-beta and TGF-beta differentially regulate IL-12 activity in human peripheral blood mononuclear cells. Immunology Letters, v. 75, n. 2, p. 117-122, 2001.
ISSN: 0165-2478
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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