Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11198
Title: Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.
Authors: Soto, Manuel
Corvo, Laura
Garde, Esther
Ramírez, Laura
Iniesta, Virginia
Bonay, Pedro
Gómez Nieto, Carlos
González, Victor M
Martín, M. Elena
Alonso, Carlos
Coelho, Eduardo Antonio Ferraz
Barral, Aldina Maria Prado
Barral Netto, Manoel
Iborra, Salvador
Affilliation: Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, Spain
Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, Spain
Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, Spain
Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, Spain
University of Extrema. Leishman Ceres Laboratory (GLP Compliance Certified) Parasitology. Caceres, Spain
Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, Spain
University of Extrema. Leishman Ceres Laboratory (GLP Compliance Certified) Parasitology. Caceres, Spain
Instituto Ralmon y Cajal de Investigacion Sanitaria (IRYCIS). Hospital Ramon y Cajal. Departamento de Bioquimica-Investigacion. Madrid, Spain
Instituto Ralmon y Cajal de Investigacion Sanitaria (IRYCIS). Hospital Ramon y Cajal. Departamento de Bioquimica-Investigacion. Madrid, Spain
Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, Spain
Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Patologia Clinica. COLTEC. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Centro Nacional de Investigaciones Cardiovasculares. Department of Vascular Biology and Inflammation. Immunobiology of Inflammation Laboratory. Madrid, Spain
Abstract: BACKGROUND: Highly conserved intracellular proteins from Leishmania have been described as antigens in natural and experimental infected mammals. The present study aimed to evaluate the antigenicity and prophylactic properties of the Leishmania infantum Poly (A) binding proteins (LiPABPs). METHODOLOGY/PRINCIPAL FINDINGS: Three different members of the LiPABP family have been described. Recombinant tools based on these proteins were constructed: recombinant proteins and DNA vaccines. The three recombinant proteins were employed for coating ELISA plates. Sera from human and canine patients of visceral leishmaniasis and human patients of mucosal leishmaniasis recognized the three LiPABPs. In addition, the protective efficacy of a DNA vaccine based on the combination of the three Leishmania PABPs has been tested in a model of progressive murine leishmaniasis: BALB/c mice infected with Leishmania major. The induction of a Th1-like response against the LiPABP family by genetic vaccination was able to down-regulate the IL-10 predominant responses elicited by parasite LiPABPs after infection in this murine model. This modulation resulted in a partial protection against L. major infection. LiPABP vaccinated mice showed a reduction on the pathology that was accompanied by a decrease in parasite burdens, in antibody titers against Leishmania antigens and in the IL-4 and IL-10 parasite-specific mediated responses in comparison to control mice groups immunized with saline or with the non-recombinant plasmid. CONCLUSION/SIGNIFICANCE: The results presented here demonstrate for the first time the prophylactic properties of a new family of Leishmania antigenic intracellular proteins, the LiPABPs. The redirection of the immune response elicited against the LiPABP family (from IL-10 towards IFN-γ mediated responses) by genetic vaccination was able to induce a partial protection against the development of the disease in a highly susceptible murine model of leishmaniasis.
keywords: Leishmania Infantum
Leishmania major
Proteinas
Vacinacao
Humanos
Issue Date: 2015
Publisher: Public Library of Science
Citation: SOTO, M. et al. Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice. PLoS Neglected Tropical Diseases, v. 9, n. 5, p. e0003751, 2015.
DOI: 10.371/journal.pntd.003751
ISSN: 1935-2735
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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