| Author | Santos, Priscila Pinto Silva dos | |
| Author | Barros, Geisa Baptista | |
| Author | Mineo, José Roberto | |
| Author | Silva, Deise Aparecida de Oliveira | |
| Author | Menegaz, Mauro Hygino Weinert | |
| Author | Serufo, José Carlos | |
| Author | Dietze, Reynaldo | |
| Author | Martins Filho, Olindo Assis | |
| Author | Lemos, Elenice Moreira | |
| Access date | 2015-08-13T14:41:36Z | |
| Available date | 2015-08-13T14:41:36Z | |
| Document date | 2012 | |
| Citation | SANTOS, Priscila Pinto Silva dos et al. Flow cytometry-based algorithm to analyze the anti-fixed Toxoplasma gondii tachyzoites IgM and IgG reactivity and diagnose human acute toxoplasmosis. Journal of Immunological Methods, vol. 378, n. 1-2, p. 33-43, 2012. | pt_BR |
| ISSN | 0022-1759 | |
| URI | https://www.arca.fiocruz.br/handle/icict/11444 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Ltd. | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Flow cytometry-based algorithm to analyze the anti-fixed Toxoplasma gondii tachyzoites IgM and IgG reactivity and diagnose human acute toxoplasmosis | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jim.2012.02.001 | |
| Abstract | In the present study we evaluated the performance of a flow cytometry-based algorithm as a new serological approach to detect antibodies to T. gondii and specific IgG avidity to diagnose acute toxoplasmosis. The results showed that using FC-AFTA-IgM assay, all serum samples from patients with acute toxoplasmosis demonstrated seropositivity, whereas 90% of patients with chronic infection and 100% of non-infected individuals presented negative results. Thus, only 10% of patients with chronic toxoplasmosis showed residual IgM, in contrast with other methodologies used to diagnosis acute toxoplasmosis. On the order hand, FC-AFTA-IgG assay as well as FC-AFTA-IgG subclasses is unlikely to discriminate acute from chronic toxoplasmosis. We have also evaluated the performance of FC-AFTA-IgG avidity as a tool to exclude chronic toxoplasmosis in patients with positive FC-AFTA-IgM. Our data showed an excellent performance of FC-AFTA-IgG avidity employing the cut-off of 60% for Avidity Index (AI) with sensitivity and specificity of 100%. All serum samples from patients presenting acute toxoplasmosis showed low avidity index (AI ≤ 60%), whereas all chronic patients showed high avidity index (AI > 60%). The outstanding performance indexes of this novel flow cytometry-based algorithm support its use as a non-conventional alternative serological approach to diagnose human acute toxoplasmosis. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES Brazil | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES Brazil/Universidade Federal de Minas Gerais. Departamento de Clínica Médica. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Laboratório de Imunoparasitologia. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Laboratório de Imunoparasitologia. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Clínica Médica. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES Brazil | pt_BR |
| Subject | Acute toxoplasmosis | pt_BR |
| Subject | Serology | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Flow cytometry | pt_BR |
