Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11444
Title: Flow cytometry-based algorithm to analyze the anti-fixed Toxoplasma gondii tachyzoites IgM and IgG reactivity and diagnose human acute toxoplasmosis
Authors: Santos, Priscila Pinto Silva dos
Barros, Geisa Baptista
Mineo, José Roberto
Silva, Deise Aparecida de Oliveira
Menegaz, Mauro Hygino Weinert
Serufo, José Carlos
Dietze, Reynaldo
Martins Filho, Olindo Assis
Lemos, Elenice Moreira
Affilliation: Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES Brazil
Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES Brazil/Universidade Federal de Minas Gerais. Departamento de Clínica Médica. Belo Horizonte, MG, Brazil
Universidade Federal de Uberlândia. Laboratório de Imunoparasitologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Laboratório de Imunoparasitologia. Uberlândia, MG, Brazil
Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES Brazil
Universidade Federal de Minas Gerais. Departamento de Clínica Médica. Belo Horizonte, MG, Brazil
Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil
Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES Brazil
Abstract: In the present study we evaluated the performance of a flow cytometry-based algorithm as a new serological approach to detect antibodies to T. gondii and specific IgG avidity to diagnose acute toxoplasmosis. The results showed that using FC-AFTA-IgM assay, all serum samples from patients with acute toxoplasmosis demonstrated seropositivity, whereas 90% of patients with chronic infection and 100% of non-infected individuals presented negative results. Thus, only 10% of patients with chronic toxoplasmosis showed residual IgM, in contrast with other methodologies used to diagnosis acute toxoplasmosis. On the order hand, FC-AFTA-IgG assay as well as FC-AFTA-IgG subclasses is unlikely to discriminate acute from chronic toxoplasmosis. We have also evaluated the performance of FC-AFTA-IgG avidity as a tool to exclude chronic toxoplasmosis in patients with positive FC-AFTA-IgM. Our data showed an excellent performance of FC-AFTA-IgG avidity employing the cut-off of 60% for Avidity Index (AI) with sensitivity and specificity of 100%. All serum samples from patients presenting acute toxoplasmosis showed low avidity index (AI ≤ 60%), whereas all chronic patients showed high avidity index (AI > 60%). The outstanding performance indexes of this novel flow cytometry-based algorithm support its use as a non-conventional alternative serological approach to diagnose human acute toxoplasmosis.
Keywords: Acute toxoplasmosis
Serology
Diagnosis
Flow cytometry
Issue Date: 2012
Publisher: Elsevier Ltd.
Citation: SANTOS, Priscila Pinto Silva dos et al. Flow cytometry-based algorithm to analyze the anti-fixed Toxoplasma gondii tachyzoites IgM and IgG reactivity and diagnose human acute toxoplasmosis. Journal of Immunological Methods, vol. 378, n. 1-2, p. 33-43, 2012.
DOI: 10.1016/j.jim.2012.02.001
ISSN: 0022-1759
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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