Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11464
Title: Activities of Psilostachyin A an Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo
Authors: Silva, Cristiane França da
Batista, Denise da Gama Jaen
Araújo, Julianna Siciliano de
Batista, Marcos Meuser
Lionel, Jessica
Souza, Ellen Mello de
Hammer, Erica Ripoll
Silva, Patricia Bernardino da
Miert, Marta de
Adams, Michael
Zimmermann, Stefanie
Hamburger, Matthias
Brun, Reto
Schühly, Wolfang
Soeiro, Maria de Nazaré Correia
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
University of Basel. Department of Pharmaceutical Sciences, Pharmaceutical Biology. Basel, Switzerland.
University of Basel. Department of Pharmaceutical Sciences, Pharmaceutical Biology. Basel, Switzerland.
University of Basel. Department of Pharmaceutical Sciences, Pharmaceutical Biology. Basel, Switzerland / Swiss Tropical and Public Health Institute. Department of Medical Parasitology and Infection Biology. Basel, Switzerland.
University of Basel. Department of Pharmaceutical Sciences, Pharmaceutical Biology. Basel, Switzerland.
Swiss Tropical and Public Health Institute. Department of Medical Parasitology and Infection Biology. Basel, Switzerland.
University of Graz. Institute of Zoology. Graz, Austria.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Abstract: In vitro and in vivo activities against Trypanosoma cruzi were evaluated for two sesquiterpene lactones: psilostachyin A and cynaropicrin. Cynaropicrin had previously been shown to potently inhibit African trypanosomes in vivo, and psilostachyin A had been reported to show in vivo effects against T. cruzi, albeit in another test design. In vitro data showed that cynaropicrin was more effective than psilostachyin A. Ultrastructural alterations induced by cynaropicrin included shedding events, detachment of large portions of the plasma membrane, and vesicular bodies and large vacuoles containing membranous structures, suggestive of parasite autophagy. Acute toxicity studies showed that one of two mice died at a cynaropicrin dose of 400 mg/kg of body weight given intraperitoneally (i.p.). Although no major plasma biochemical alterations could be detected, histopathology demonstrated that the liver was the most affected organ in cynaropicrin-treated animals. Although cynaropicrin was as effective as benznidazole against trypomastigotes in vitro, the treatment (once or twice a day) of T. cruzi-infected mice (up to 50 mg/kg/day cynaropicrin) did not suppress parasitemia or protect against mortality induced by the Y and Colombiana strains. Psilostachyin A (0.5 to 50 mg/kg/day given once a day) was not effective in the acute model of T. cruzi infection (Y strain), reaching 100% animal mortality. Our data demonstrate that although it is very promising against African trypanosomes, cynaropicrin does not show efficacy compared to benznidazole in acute mouse models of T. cruzi infection.
Keywords: Trypanosoma cruzi
In Vitro
In Vivo
Psilostachyin A
Cynaropicrin
keywords: Medicamentos
DeCS: Técnicas In Vitro
Trypanosoma cruzi
Issue Date: 2013
Publisher: American Society for Microbiology
Citation: SILVA, Cristiane França da; et al. Activities of Psilostachyin A and Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo. Antimicrobial Agents and Chemotherapy, v.57, n.11, p.5307-5314, nov. 2013.
DOI: 10.1128/AAC.00595-13
ISSN: 0066-4804
1098-6596
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
fabiana_rocha_etal_IOC-2013.pdf7.81 MBAdobe PDFView/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.