Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11465
Title: Trypanosoma cruzi Infection in Neotropical Wild Carnivores (Mammalia: Carnivora): At the Top of the T. cruzi Transmission Chain
Authors: Rocha, Fabiana Lopes
Roque, André Luiz Rodrigues
Lima, Juliane Saab de
Cheida, Carolina Carvalho
Lemos, Frederico Gemesio
Azevedo, Fernanda Cavalacanti de
Arrais, Ricardo Corassa
Bilac, Daniele
Herrera, Heitor Miraglia
Mourão, Guilherme
Jansen, Ana Maria
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil / Instituto Brasileiro para Medicina da Conservação, TRÍADE. Recife, PE, Brasil / Programa de Conservação Mamíferos do Cerrado, PCMC. Araguari, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.
Universidade Federal de Mato Grosso do Sul - UFMS. Programa de Pós-graduação em Ecologia e Conservação. Campo Grande, MS, Brasil.
Instituto de Pesquisas Cananéia (IPeC). Programa de Pesquisa em Vida Selvagem/Projeto Carnívoros. Cananéia, SP, Brasil.
Programa de Conservação Mamíferos do Cerrado, PCMC. Araguari, MG, Brasil / Universidade Federal de Goiás. Departamento de Ciências Biológicas. Catalão, GO, Brasil.
Programa de Conservação Mamíferos do Cerrado, PCMC. Araguari, MG, Brasil.
Programa de Conservação Mamíferos do Cerrado, PCMC. Araguari, MG, Brasil / Universidade de São Paulo.Departamento de Medicina Preventiva e Saúde Animal . São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.
Universidade Católica Dom Bosco - UCDB. Campo Grande, MS, Brasil.
Empresa Brasileira de Pesquisa Agropecuária, Embrapa Pantanal. Laboratório de Vida Selvagem. Corumbá, MS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.
Abstract: Little is known on the role played by Neotropical wild carnivores in the Trypanosoma cruzi transmission cycles. We investigated T. cruzi infection in wild carnivores from three sites in Brazil through parasitological and serological tests. The seven carnivore species examined were infected by T. cruzi, but high parasitemias detectable by hemoculture were found only in two Procyonidae species. Genotyping by Mini-exon gene, PCR-RFLP (1f8/Akw21I) and kDNA genomic targets revealed that the raccoon (Procyon cancrivorus) harbored TcI and the coatis (Nasua nasua) harbored TcI, TcII, TcIII-IV and Trypanosoma rangeli, in single and mixed infections, besides four T. cruzi isolates that displayed odd band patterns in the Mini-exon assay. These findings corroborate the coati can be a bioaccumulator of T. cruzi Discrete Typing Units (DTU) and may act as a transmission hub, a connection point joining sylvatic transmission cycles within terrestrial and arboreal mammals and vectors. Also, the odd band patterns observed in coatis’ isolates reinforce that T. cruzi diversity might be much higher than currently acknowledged. Additionally, we assembled our data with T. cruzi infection on Neotropical carnivores’ literature records to provide a comprehensive analysis of the infection patterns among distinct carnivore species, especially considering their ecological traits and phylogeny. Altogether, fifteen Neotropical carnivore species were found naturally infected by T. cruzi. Species diet was associated with T. cruzi infection rates, supporting the hypothesis that predator-prey links are important mechanisms for T. cruzi maintenance and dispersion in the wild. Distinct T. cruzi infection patterns across carnivore species and study sites were notable. Musteloidea species consistently exhibit high parasitemias in different studies which indicate their high infectivity potential. Mesocarnivores that feed on both invertebrates and mammals, including the coati, a host that can be bioaccumulator of T. cruzi DTU’s, seem to take place at the top of the T. cruzi transmission chain.
Keywords: Trypanosoma cruzi
Mammalia
Wild Carnivores
Brazil
keywords: Brasil
DeCS: Trypanosoma cruzi
Mamíferos
Carnívoros
Issue Date: 2013
Publisher: Plos One
Citation: ROCHA, Fabiana Lopes; et al. Trypanosoma cruzi Infection in Neotropical Wild Carnivores (Mammalia: Carnivora): At the Top of the T. cruzi Transmission Chain. Plos One, v.8, n.7, 120, july 2013.
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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