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INTERROGATING THE VENOM OF THE VIPERID SNAKE SISTRURUS CATENATUS EDWARDSII BY A COMBINED APPROACH OF ELECTROSPRAY AND MALDI MASS SPECTROMETRY
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Espectrometria de Massas por Ionização por Electrospray
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / National University of Singapore. Department of Biological Sciences. Singapore.
National University of Singapore. Department of Biological Sciences. Singapore.
University of Northern Colorado. School of Biological Sciences. Greeley, Colorado, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
National University of Singapore. Department of Biological Sciences. Singapore.
National University of Singapore. Department of Biological Sciences. Singapore / Virginia Commonwealth University. Medical College of Virginia. Department of Biochemistry and Molecular Biology. Richmond, Virginia, USA.
National University of Singapore. Department of Biological Sciences. Singapore.
University of Northern Colorado. School of Biological Sciences. Greeley, Colorado, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
National University of Singapore. Department of Biological Sciences. Singapore.
National University of Singapore. Department of Biological Sciences. Singapore / Virginia Commonwealth University. Medical College of Virginia. Department of Biochemistry and Molecular Biology. Richmond, Virginia, USA.
Abstract
The complete sequence characterization of snake venom proteins by mass spectrometry is rather challenging due to the presence of multiple isoforms from different protein families. In the present study, we investigated the tryptic digest of the venom of the viperid snake Sistrurus catenatus edwardsii by a combined approach of liquid chromatography coupled to either electrospray (online) or MALDI (offline) mass spectrometry. These different ionization techniques proved to be complementary allowing the identification a great variety of isoforms of diverse snake venom protein families, as evidenced by the detection of the corresponding unique peptides. For example, ten out of eleven predicted isoforms of serine proteinases of the venom of S. c. edwardsii were distinguished using this approach. Moreover, snake venom protein families not encountered in a previous transcriptome study of the venom gland of this snake were identified. In essence, our results support the notion that complementary ionization techniques of mass spectrometry allow for the detection of even subtle sequence differences of snake venom proteins, which is fundamental for future structure-function relationship and possible drug design studies.
DeCS
SerpentesEspectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Espectrometria de Massas por Ionização por Electrospray
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