Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11617
Title: Morphological aspects of Angiostrongylus costaricensis by light andscanning electron microscopy
Authors: Rebello, Karina M.
Menna-Barreto, Rubem F. S.
Moutinho, Vanessa A. Chagas
Mota, Ester M.
Perales, Jonas
Ferreira, Ana Gisele C. Neves
Menezes, Aleksandra Oliveira
Lenzi, Henrique
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biologia de Helmintos Otto Wucherer. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro - Campus Macaé. Grupo de Sistemática e Biologia Evolutiva (GSE). Macaé, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.
Abstract: tAngiostrongylus costaricensis is a parasitic nematode that can cause severe gastrointestinal disease,known as abdominal angiostrongiliasis, in humans. This paper presents the characterization of first-and third-stage larvae and male and female adult worms of A. costaricensis by scanning electron andlight microscopy. Several novel anatomical structures were identified by scanning electron microscopy,including details of the cuticular striations of the spicules in male worms and a protective flap of the cuti-cle covering the vulvar aperture in female worms. Other taxonomic features revealed by light microscopyinclude the gubernaculum and the esophageal–intestinal valve. The use of two microscopy techniquesallowed a detailed characterization of the morphology of this nematode. A number of previously identi-fied taxonomic features, such as the striated nature of the spicules and the lateral alae were confirmed;however, the use of scanning electron microscopy resulted in a reassessment of the correct number ofpapillae distributed around the oral opening and behind the cloacal opening. These observations, in com-bination with light microscopy-based characterization of the gubernaculum and esophageal valves, haveallowed a more detailed description of this nematode taxonomy.
Keywords: Angiostrongylus costaricensis
Morphology
Light microscopy
Scanning electron microscopy
DeCS: Microscopia Eletrônica de Varredura
Infecções por Nematoides
Gastroenteropatias
Issue Date: 2013
Publisher: Elsevier
Citation: REBELLO, Karina M.; et al. Morphological aspects of Angiostrongylus costaricensis by light andscanning electron microscopy. Acta Tropica, v.127, n.3, p.191– 198, 2013.
DOI: 10.1016/j.actatropica.2013.05.002
ISSN: 0001-706X
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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