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HUMAN T-CELL AND ANTIBODY MEDIATED RESPONSES TO THE MYCOBACTERIUM TUBERCULOSIS RECOMBINANT 85A, 85B AND ESAT-6 ANTIGENS
Macedo, Gilson Costa et al. | Date Issued: 2011
Author
Macedo, Gilson Costa
Melo, Adriana Bozzi de
Weinreich, Helena Rachel
Bafica, Andre Luiz Barbosa
Teixeira, Henrique Couto
Oliveira, Sergio Costa
Affilliation
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquimica e Imunologia. Laboratorio de Imunologia e Doenças Infecciosas. Belo Horizonte, MG, Brazil/ Universidade Federal de Santa Catarina. Departamento de Parasitologia, Microbiologia e Imunologia. Florianopolis, SC, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquimica e Imunologia. Laboratorio de Imunologia e Doenças Infecciosas. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil
Universidade Federal de Santa Catarina. Departamento de Parasitologia, Microbiologia e Imunologia. Florianopolis, SC, Brazil
Universidade Federal de Juiz de Fora. Instituto de Ciencias Biologicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquimica e Imunologia. Laboratorio de Imunologia e Doenças Infecciosas. Belo Horizonte, MG, Brazil
Abstract
Tuberculosis remains a major health problem throughout the world causing large number of deaths. Effective disease control and eradication programs require the identification of major antigens recognized by the protective responses against M. tuberculosis. In this study, we have investigated humoral and cellular immune responses to M. tuberculosis-specific Ag85A, Ag85B, and ESAT-6 antigens in Brazilian patients with pulmonary (P, n = 13) or extrapulmonary (EP, n = 12) tuberculosis, patients undergoing chemotherapy (PT, n = 23), and noninfected healthy individuals (NI, n = 7). Compared to NI, we observed increased levels of IgG1 responses to Ag85B and ESAT-6 in P and PT groups. Regarding cellular immunity, Ag85A and ESAT-6 were able to discriminate P, PT, and EP patients from healthy individuals by IFN-γ production and P and PT groups from EP individuals by production of TNF-α. In summary, these findings demonstrate the ability of Ag85A, Ag85B, and ESAT-6 to differentiate TB patients from controls by IgG1, IFN-γ and TNF-α production.
Keywords
Tuberculosis
Publisher
Wiley Online Library
Citation
MACEDO, Gilson Costa et al. Human T-cell and antibody mediated responses to the Mycobacterium tuberculosis recombinant 85A, 85B and ESAT-6 antigens. Clinical & Developmental Immunology, vol. 2011, ID 351573, 2011.
DOI
10.1155/2011/351573
ISSN
1365-2249