Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11676
Title: A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice
Authors: Carlétti, Dyego
Fonseca, Denise Morais da
Gembre, Ana Flavia
Masson, Ana Paula
Campos, Lívia Weijenborg
Leite, Luciana C. C.
Pires, Andréa Rodrigues
Vieira, Joseli Lannes
Silva, Célio Lopes
Bonato, Vânia Luiza Deperon
Horn, Cynthia
Affilliation: Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Imunologia e Imunogenética. Rio de Janeiro, RJ, Brasil.
Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.
Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.
Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.
Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.
Instituto Butantã. Centro de Biotecnologia. São Paulo, SP, brasil.
Fonte Medicina Diagnóstica. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, RJ, Brasil.
Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.
Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Imunologia e Imunogenética. Rio de Janeiro, RJ, Brasil
Abstract: Mycobacterium bovis BCG prime DNA (Mycobacterium tuberculosis genes)-booster vaccinations have been shown to induce greater protection against tuberculosis (TB) than BCG alone. This heterologous prime-boost strategy is perhaps the most realistic vaccination for the future of TB infection control, especially in countries where TB is endemic. Moreover, a prime-boost regimen using biodegradable microspheres seems to be a promising immunization to stimulate a long-lasting immune response. The alanine proline antigen (Apa) is a highly immunogenic glycoprotein secreted by M. tuberculosis. This study investigated the immune protection of Apa DNA vaccine against intratracheal M. tuberculosis challenge in mice on the basis of a heterologous prime-boost regimen. BALB/c mice were subcutaneously primed with BCG and intramuscularly boosted with a single dose of plasmid carrying apa and 6,6=-trehalose dimycolate (TDM) adjuvant, coencapsulated in microspheres (BCG-APA), and were evaluated 30 and 70 days after challenge. This prime-boost strategy (BCG-APA) resulted in a significant reduction in the bacterial load in the lungs, thus leading to better preservation of the lung parenchyma, 70 days postinfection compared to BCG vaccinated mice. The profound effect of this heterologous prime-boost regimen in the experimental model supports its development as a feasible strategy for prevention of TB.
Keywords: Tuberculosis
DNA Vaccine
Mycobacterium bovis
Mycobacterium bovis BCG prime DNA
DeCS: Tuberculose
Mycobacterium bovis
Vacina BCG
Issue Date: 2013
Publisher: American Society for Microbiology
Citation: CARLÉTTI, Dyego; et al. A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice. Clinical and Vaccine Immunology, v.20, n,8, p.1162-1169, Aug. 2013.
DOI: 10.1128/CVI.00148-13
Copyright: open access
Appears in Collections:Fiocruz Mata Atlântica - Artigos de Periódicos
INI - Artigos de Periódicos
IOC - Artigos de Periódicos

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