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HUMAN G9P[8] ROTAVIRUS STRAINS CIRCULATING IN CAMEROON, 1999–2000: GENETIC RELATIONSHIPS WITH OTHER G9 STRAINS AND DETECTION OF A NEW G9 SUBTYPE
Esona, M. D. et al. | Date Issued: 2013
Author
Esona, M. D.
Mijatovic-Rustempasic, S.
Foytich, K.
Roy, S.
Banyai, K.
Armah, G. E.
Steele, A. D.
Volotão, E. M.
Gomez, M. M.
Silva, M. F. M.
Gautam, R.
Quaye, O.
Tam, K. I.
Forbi, J. C.
Seheri, M.
Page, N.
Nyangao, J.
Ndze, V. N.
Aminu, M.
Bowen, M. D.
Gentsch, J. R.
Affilliation
Centers for Disease Control and Prevention. Gastroenteritis and Respiratory Viruses Laboratory Branch. Atlanta, USA / University of Limpopo. Medunsa Campus. Department of Virology. MRC/UL Diarrhoeal Pathogens Research Unit. South Africa.
Centers for Disease Control and Prevention. Gastroenteritis and Respiratory Viruses Laboratory Branch. Atlanta, USA.
Centers for Disease Control and Prevention. Gastroenteritis and Respiratory Viruses Laboratory Branch. Atlanta, USA.
Centers for Disease Control and Prevention. Gastroenteritis and Respiratory Viruses Laboratory Branch. Atlanta, USA.
Hungarian Academy of Sciences. Centre for Agricultural Research. Institute for Veterinary Medical Research. Budapest, Hungary.
University of Ghana. College of Health Sciences/Noguchi Memorial Research Institute Accra. Legon, Ghana.
PATH, 1455 Leary Way, NW, Seattle, WA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Centers for Disease Control and Prevention. Gastroenteritis and Respiratory Viruses Laboratory Branch. Atlanta, USA.
Centers for Disease Control and Prevention. Gastroenteritis and Respiratory Viruses Laboratory Branch. Atlanta, USA.
Centers for Disease Control and Prevention. Gastroenteritis and Respiratory Viruses Laboratory Branch. Atlanta, USA.
Innovative Biotech. Virology Laboratory. Keffi/Abuja, Nigeria.
University of Limpopo. Medunsa Campus. Department of Virology. MRC/UL Diarrhoeal Pathogens Research Unit. South Africa.
University of Limpopo. Medunsa Campus. Department of Virology. MRC/UL Diarrhoeal Pathogens Research Unit. South Africa / National Institute for Communicable Diseases. Viral Gastroenteritis Unit. South Africa.
Kenya Medical Research Institute. Centre for Virus Research. Nairobi, Kenia.
University of Yaoundé I.. Faculty of Medicine and Biomedical Sciences. Yaoundé, Cameron.
Ahmadu Bello University. Department of Microbiology. Zaria, Nigeria.
Centers for Disease Control and Prevention. Gastroenteritis and Respiratory Viruses Laboratory Branch. Atlanta, USA.
Centers for Disease Control and Prevention. Gastroenteritis and Respiratory Viruses Laboratory Branch. Atlanta, USA.
Abstract
Group A rotaviruses (RV-A) are the leading cause of viral gastroenteritis in children worldwide and genotype G9P[8] is one of the five most common genotypes detected in humans. In order to gain insight into the degree of genetic variability of G9P[8] strains circulating in Cameroon, stool samples were collected during the 1999–2000 rotavirus season in two different geographic regions in Cameroon (Southwest and Western Regions). By RT-PCR, 15 G9P[8] strains (15/89 = 16.8%) were identified whose genomic configurations was subsequently determined by complete or partial gene sequencing. In general, all Cameroonian G9 strains clustered into current globally-spread sublineages of the VP7 gene and displayed 86.6– 100% nucleotide identity amongst themselves and 81.2–99.5% nucleotide identity with global G9 strains. The full genome classification of all Cameroonian strains was G9-P[8]-I1–R1–C1–M1–A1–N1–T1–E1–H1 but phylogenetic analysis of each gene revealed that the strains were spread across 4 or more distinct lineages. An unusual strain, RVA/Human-wt/CMR/6788/1999/G9P[8], which shared the genomic constellation of other Cameroonian G9P[8] strains, contained a novel G9 subtype which diverged significantly (18.8% nucleotide and 19% amino acid distance) from previously described G9 strains. Nucleotide and amino acid alignments revealed that the 30 end of this gene is highly divergent from other G9 VP7 genes suggesting that it arose through extensive accumulation of point mutations. The results of this study demonstrate that diverse G9 strains circulated in Cameroon during 1999–2000.
Keywords
Rotavirus A
Genotype P[8]G9
Genomic phylogenetic analysis
Structural proteins
Non-structural proteins
Ca,eroon
 
DeCS
Rotavirus
Genética
Gastroenterite
Infecções por Rotavirus
 
Publisher
Elsevier
Citation
ESONA, m. d.; et al.Human G9P[8] rotavirus strains circulating in Cameroon, 1999–2000: Genetic relationships with other G9 strains and detection of a new G9 subtype. Infection, Genetics and Evolution, v.18, p.315-324, Aug. 2013.
DOI
10.1016/j.meegid.2013.06.005
ISSN
1567-1348