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GENOTYPIC CHARACTERIZATION OF YERSINIA ENTEROCOLITICA BIOTYPE 4/O:3 ISOLATES FROM PIGS AND SLAUGHTERHOUSES USING SE-AFLP, ERIC-PCR, AND PFGE
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Abstract
Yersinia enterocolitica is a foodborne pathogen that causes illness in humans and animals. The biotype 4/O:3 has been commonly
associated with yersiniosis and is characterized by the presence of chromosomal and extra-chromosomal virulence genes.Molecular
typing methods have been successfully used to characterize Y. enterocolitica genetic heterogeneity and to study the epidemiology of
the bacteria from different origins. In this study, 320 Y. enterocolitica biotype 4/O:3 isolates originating in pigs and slaughterhouses
were characterized according to the virulence profile, and 61 isolates were typified through SE-AFLP, ERIC-PCR, and PFGE
techniques. The majority of the isolates originated from pigs, and the predominant virulence profile was ail+ virF+ rfbC+ ystA+,
representing 83.4% of the tested isolates. All of the Y. enterocolitica 4/O:3 isolates were positive for at least ystA gene.The SE-AFLP
and ERIC-PCR patterns were highly homogeneous. The SE-AFLP was more discriminative than the ERIC-PCR and tended to
cluster isolates according to the slaughterhouse. Despite the limited genetic diversity of Y. enterocolitica 4/O:3, PFGE was shown to
be the most discriminative technique considering one band of difference. Fattening pigs proved to be an important reservoir of Y.
enterocolitica biotype 4/O:3 carrying virulence genes.
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