Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11803
Title: Canine Cutaneous Leishmaniasis: Dissemination and Tissue Tropism of Genetically Distinct Leishmania (Viannia) braziliensis Populations
Authors: Oliveira, Guilherme Marx de
Madeira, Maria de Fátima
Oliveira, Fernanda Santos
Pires, Marize Quinhones
Pacheco, Raquel da Silva
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratóorio de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clíınica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratóorio de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratóorio de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratóorio de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.
Abstract: Little is known regarding the internal dissemination of initial cutaneous lesions and tissue tropism of Leishmania (Viannia) braziliensis populations in naturally infected dogs. The aim of this study was to investigate genetic polymorphisms of L. (V.) braziliensis populations in different anatomic sites of naturally infected dogs by using polymerase chain reaction (PCR) and lowstringency single specific primer-PCR (LSSP-PCR) techniques. The amplified products were analyzed by LSSP-PCR to investigate the genetic variability of the parasite populations present in different anatomical sites. Twenty-three out of the 52 samples gave PCR-positive results. The existence of L. (V.) braziliensis strains that remained restricted to cutaneous lesions and others showing characteristics of dissemination to internal organs and healthy skin was observed. LSSP-PCR and numerical analyses revealed that parasite populations that do not disseminate were genetically similar and belonged to a separate phenetic cluster. In contrast, populations that showed spreading to internal organs displayed a more polymorphic genetic profile. Despite the heterogeneity, L. (V.) braziliensis populations with identical genetic profiles were observed in popliteal and cervical lymph nodes of the same animal. Our results indicate that infection in dogs can bemanifested by dissemination and tissue tropism of genetically distinct populations of L. (V.) braziliensis.
Keywords: Leishmaniasis
Cutaneous leishmaniasis (CL)
Leishmaniasis (VL)
Canine
Tissue Tropism
DeCS: Leishmaniose
Visceral leishmaniose
Tropismo
Issue Date: 2013
Publisher: Hindawi Publishing Corporation
Citation: OLIVEIRA, Guilherme Marx de; et al. Canine Cutaneous Leishmaniasis: Dissemination and Tissue Tropism of Genetically Distinct Leishmania (Viannia) braziliensis Populations. Veterinary Medicine International, v.2013, Article ID 982183, 5p, 2013.
DOI: 10.1155/2013/982183
ISSN: 2042-0048
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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