Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11810
Title: Ilheus Virus Isolation in the Pantanal, West-Central Brazil
Authors: Corrêa, Alex Pauvolid
Kenney, Joan L.
Lima, Dinair Couto
Campos, Zilca M. S.
Schatzmayr, Hermann G.
Nogueira, Rita M. R.
Brault, Aaron C.
Komar, Nicholas
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil / Centers for Disease Control and Prevention. Arbovirus Diseases Branch. Fort Collins, Colorado, USA / Fulbright Visiting Researcher in Doctorate Sandwich Program at CDC. Fort Collins, Colorado, USA.
Centers for Disease Control and Prevention. Arbovirus Diseases Branch. Fort Collins, Colorado, USA .
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Embrapa Pantanal. Corumbá, MS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Centers for Disease Control and Prevention. Arbovirus Diseases Branch. Fort Collins, Colorado, USA .
Fulbright Visiting Researcher in Doctorate Sandwich Program at CDC. Fort Collins, Colorado, USA.
Abstract: The wetlands of the Brazilian Pantanal host large concentrations of diverse wildlife species and hematophagous arthropods, conditions that favor the circulation of zoonotic arboviruses. A recent study from the Nhecolaˆndia sub-region of Pantanal reported serological evidence of various flaviviruses, including West Nile virus and Ilheus virus (ILHV). According to the age of seropositive horses, at least three flaviviruses, including ILHV, circulated in the Brazilian Pantanal between 2005 and 2009. To extend this study, we collected 3,234 adult mosquitoes of 16 species during 2009 and 2010 in the same sub-region. Mosquito pool homogenates were assayed for infectious virus on C6/36 and Vero cell monolayers and also tested for flaviviral RNA by a group-specific real-time RT-PCR. One pool containing 50 non-engorged female specimens of Aedes scapularis tested positive for ILHV by culture and for ILHV RNA by real-time RT-PCR, indicating a minimum infection rate of 2.5 per 1000. Full-length genomic sequence exhibited 95% identity to the only full genome sequence available for ILHV. The present data confirm the circulation of ILHV in the Brazilian Pantanal.
Keywords: Hematophagous arthropods
Brazilian Pantanal
Wildlife species
Zoonotic arboviruses
Flaviviruses
ILHV RNA
keywords: Flavivírus
Pantanal
Ilheus virus (ILHV
Issue Date: 2013
Publisher: Plos One
Citation: CORRÊA, Alex Pauvolid; et al. Ilheus Virus Isolation in the Pantanal, West-Central Brazil. PLoS Negl Trop Dis, v.7, n.7, : e2318, 8p, July 2013.
DOI: 10.1371/journal.pntd.0002318
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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