Author | Galeano, Maria Eugenia | |
Author | Martinez, Magaly | |
Author | Amarilla, Alberto A. | |
Author | Russomando, Graciela | |
Author | Miagostovich, Marize Pereira | |
Author | Parra, Gabriel I. | |
Author | Leite, José Paulo | |
Access date | 2015-10-02T12:45:06Z | |
Available date | 2015-10-02T12:45:06Z | |
Document date | 2013 | pt_BR |
Citation | GALEANO, Maria Eugenia; et al.Molecular epidemiology of norovirus strains in Paraguayan childrenduring 2004–2005: Description of a possible new GII.4 cluster. Journal of Clinical Virology, v.58, n.2, p.378-384, Oct. 2013. | pt_BR |
ISSN | 1386-6532 | pt_BR |
ISSN | 10.1016/j.jcv.2013.07.008 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/11869 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Crianças | pt_BR |
Subject in Portuguese | Paraguai | pt_BR |
Title | Molecular epidemiology of norovirus strains in Paraguayan childrenduring 2004–2005: Description of a possible new GII.4 cluster | pt_BR |
Type | Article | pt_BR |
Abstract | tBackground: Noroviruses (NoV) have been shown to be an important cause of morbidity and mortalityin children worldwide, only second after Group A rotaviruses (RVA). In Paraguay, acute gastroenteritis(AGE) is the third cause of mortality in children ≤5 years old.Objectives: To analyze the presence and diversity of NoV in Paraguayan children ≤5 years old presentingAGE.Study design: Three hundred seventy eight fecal samples, negative for pathogenic bacteria and RVA, werecollected from children admitted as ambulatory and hospitalized patients in a large private hospitalfrom Asuncion, Paraguay from 2004 to 2005. The presence and diversity of NoV was determined by twodifferent RT-PCR strategies and nucleotide sequencing.Results: One hundred and sixty one samples were positive for NoV by partial amplification of the viralpolymerase gene (RdRp). No seasonality or differences in the viral prevalence for the different age-groupswere detected. GII and GI NoVs were associated to 58% and 42% of the infections, respectively. The geno-type was determined in 18% (29/161) NoV-positive samples. The genotypes detected were: GII.4 (18%),GII.17 (18%), GII.6 (14%), GII.7 (14%), GII.3 (10%), GII.5 (3%), GII.8 (3%), GII.16 (3%), GI.3 (14%) and GI.8 (3%).Amplification of the ORF2 from the GII.4 strains showed the presence of a new GII.4 variant.Conclusions: The results showed a continuous circulation of NoV in children throughout the two years ofstudy and an extensive diversity of genotypes co-circulating, highlighting the need for better surveillanceof NoV in Paraguayan children. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil / National University of Asunción. Health Sciences Research Institute. Molecular Biology and Genetics Department. Asunción, Paraguay. | pt_BR |
Affilliation | National University of Asunción. Health Sciences Research Institute. Molecular Biology and Genetics Department. Asunción, Paraguay. | pt_BR |
Affilliation | National University of Asunción. Health Sciences Research Institute. Molecular Biology and Genetics Department. Asunción, Paraguay. | pt_BR |
Affilliation | National University of Asunción. Health Sciences Research Institute. Molecular Biology and Genetics Department. Asunción, Paraguay. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | National University of Asunción. Health Sciences Research Institute. Molecular Biology and Genetics Department. Asunción, Paraguay. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Norovirus | pt_BR |
Subject | Acute gastroenteritis | pt_BR |
Subject | Paraguayan children | pt_BR |
Subject | GII.4 norovirus | pt_BR |
DeCS | Norovirus | pt_BR |
DeCS | Gastroenterite | pt_BR |