Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11986
Title: Evaluation of the recombinant LipL32 in enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis.
Authors: Bomfim, Maria Rosa Quaresma
Ko, Albert Icksang
Koury, Matilde Cota
Affilliation: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Weill Medical College of Cornell University. Division of International Medicine and Infectious Diseases. New York, USA
Abstract: The recombinant leptospiral protein LipL32 was evaluated for use in the diagnosis of bovine leptospirosis by enzyme-linked immunosorbent assay (rLipL32 IgG ELISA). The microscopic agglutination test (MAT) of 150 serum samples from cattle suspected of leptospirosis showed that 125 (83.3%) samples had positive reciprocal agglutination titres, which ranged from 100 to 1600. The highest titres were observed for the serovars Hardjoprajitno and Bratislava. In the rLipL32 IgG ELISA, 83.3% of the samples were positive. The sensitivity of IgG ELISA for 125 bovine sera, which had MAT titres of greater than or equal to 100, was 100%. ELISA showed a specificity of 100% with 58 bovine sera, which were negative at a 1:50 dilution in the MAT for Leptospira interrogans serovars. When analytical specificity of the IgG ELISA was evaluted using 60 bovine serum samples from animals showing serum antibodies to other pathogens that cause abortion in cattle, such as Babesia sp., Anaplasma sp. and Brucella sp. and no cross-reaction was observed. The recombinant LipL32 IgG ELISA can be an alternative to the MAT for diagnosis of leptospiral infection in cattle.
Keywords: Leptospira
rLipL32 IgG ELISA
MAT
DeCS: Proteínas da Membrana Bacteriana Externa/química
Doenças dos Bovinos/microbiologia
Ensaio de Imunoadsorção Enzimática/veterinária
Leptospira/isolamento & purificação
Leptospirose/veterinária
Lipoproteínas/química
Testes de Aglutinação/veterinária
Animais
Anticorpos Antibacterianos/sangue
Bovinos
Doenças dos Bovinos/sangue
Ensaio de Imunoadsorção Enzimática/métodos
Feminino
Leptospirose/sangue
Proteínas Recombinantes/química
Sensibilidade e Especificidade
Issue Date: 2005
Publisher: Elsevier
Citation: BOMFIM, M. R. Q.; KO, A. I.; KOURY, M. C. Evaluation of the recombinant LipL32 in enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis. Veterinary Microbiology, v. 109, n. 1-2, p. 89-94, 2005.
DOI: 10.1016/j.vetmic.2005.05.002
ISSN: 0378-1135
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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