Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12055
Title: Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil
Authors: Pereira, Polyana Silva
Borghi, Mirla
Albano, Rodolpho Mattos
Lopes, Jonathan Christian Oliveira
Silveira, Melise Chaves
Marques, Elizabeth Andrade
Oliveira, Jane Cleide Ribeiro
Asensi, Marise Dutra
Assef, Ana Paula D’Alincourt Carvalho
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro (UERJ). Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro (UERJ). Faculdade de Ciências Médicas. Departamento de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil.
Laboratório Central de Saúde Pública do Rio de Janeiro Noel Nutels (LACEN/RJ). Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.
Abstract: The most important resistance mechanism against b-lactam drugs is the production of carbapenemases. In this study, we report the first identification of Klebsiella pneumoniae carbapenemase (KPC)-2 and New Delhi metallo-b-lactamase (NDM)-1 in Enterobacter hormaechei subps. oharae from Brazil. The detection of carbapenemases was done by phenotypic assays, PCR, and DNA sequencing, whereas the identification was performed by conventional techniques, sequencing of the 16S rDNA gene, and hsp60-genotyping. Molecular typing was performed using pulsed-field gel electrophoresis, and antimicrobial susceptibility was surrogated by the Etest methodology. Using the whole genome sequencing approach, we searched for resistance genes, plasmid incompatibility group genes, and the genetic environment of blaNDM and blaKPC. The plasmid identification was done by restriction digests with the S1 nuclease followed by hybridization using digoxigenin labeled specific probes. The isolate was considered multiresistant, being susceptible to amikacin and polymyxin B. We observed the following resistance genes: blaCTX-M-15, blaACT-7, blaTEM-1, blaOXA-1, aadA1, aadA2, strA, strB, aac(3)-II, qnrB1, and aac(6¢)-Ib-cr and incompatibility group plasmid genes IncA/C, IncHI2, and IncN. The blaKPC gene was found associated to the transposon Tn4401 isoform b in plasmid with 50 kb (IncN) and blaNDM-1 was flanked by a truncated ISAba125 and bleMBL in plasmid with 160 kb (IncA/C). This study showed the coproduction of two important carbapenemases (KPC-2 and NDM-1) associated with mobile genetic elements of worldwide epidemiological importance (Tn4401 and ISAba125, respectively), reinforcing the idea that urgent measures are necessary to reduce and prevent the spreading of these carbapenemases primarily in the hospital settings.
Keywords: Enterobacter hormaechei
KPC-2
Klebsiella pneumoniae
Brazil
NDM-1
DeCS: Enterobacter
Klebsiella pneumoniae
Brasil
Issue Date: 2015
Publisher: Mary Ann Liebert, Inc. publishers
Citation: PEREIRA, Polyana Silva; et al. Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil. Microbial Drug Resistance, v.21, n.2, p.234-236, Apr. 2015.
DOI: 10.1089/mdr.2014.0171
ISSN: 1931-8448
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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