| Author | Lima, Monique da Rocha Queiroz | |
| Author | Nogueira, Rita Maria Ribeiro | |
| Author | Fillipis, Ana Maria Bispo de | |
| Author | Nunes, Priscila Conrado Guerra | |
| Author | Sousa, Carla Santos de | |
| Author | Silva, Manoela Heringer da | |
| Author | Santos, Flávia Barreto dos | |
| Access date | 2015-10-23T16:14:07Z | |
| Available date | 2015-10-23T16:14:07Z | |
| Document date | 2014 | |
| Citation | LIMA, Monique da Rocha Queiroz et al. A simple heat dissociation method increases significantly the ELISA detection sensitivity of the nonstructural-1 glycoprotein in patients infected with DENV type-4. Journal of Virological Methods, v. 204, p.105-108, 2014. | pt_BR |
| ISSN | 0166-0934 | |
| URI | https://www.arca.fiocruz.br/handle/icict/12078 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Dengue | pt_BR |
| Title | A simple heat dissociation method increases significantly the ELISA detection sensitivity of the nonstructural-1 glycoprotein in patients infected with DENV type-4 | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jviromet.2014.02.031 | |
| Abstract | The secreted form of the dengue virus (DENV) nonstructural-1 (NS1) glycoprotein has been shown to be
useful for the diagnosis of DENV infections in patients’ serum samples. In a number of studies, the sensitivity
of the commercially available DENV NS1 glycoprotein detection assays was higher against some
DENV serotypes (DENV-1 > DENV-3 > DENV-2 = DENV-4) than others and were also lower using patients’
serum samples with secondary versus primary DENV infections. In this study, 471 DENV-4 positive acute
phase patients’ serum samples were selected from a large panel collected in Brazil from March 2011
to October 2012 by RT-PCR and/or virus isolation followed by serotype determination. The sera from
primary (n = 228) and secondary (n = 238) DENV-4 infections were identified using IgM and IgG capture
ELISAs. The sensitivity of a commercial DENV NS1 glycoprotein detection ELISA was then assessed when
these serum samples were not pre-treated or pre-treated by acid or heat dissociation prior to being tested.
Acid and heat dissociation of patients’ serum samples with primary and secondary DENV-4 infections
increased significantly the sensitivity of the DENV NS1 glycoprotein detection ELISA from 54.4% to 77.2%
(p < 0.05) and 82% (p < 0.05) and from 39.1% to 63.9% (p < 0.05) and 73.1% (p < 0.05), respectively. Treatment
of DENV infected patients’ serum samples using simple and rapid heat dissociation step (100 ◦C for
5 min) was, therefore, shown to be very useful for increasing the sensitivity of the DENV NS1 glycoprotein
detection ELISA using serum samples from either primary or secondary DENV infected patients. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Dengue diagnosis | pt_BR |
| Subject | NS1 ELISA | pt_BR |
| Subject | Immune-complex dissociation | pt_BR |
| Subject | DENV-4 | pt_BR |
| Subject | secondary infections | pt_BR |
| DeCS | Dengue | pt_BR |
| DeCS | Diagnóstico | pt_BR |
| DeCS | Ensaio de Imunoadsorção Enzimática | pt_BR |
