Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12078
Title: A simple heat dissociation method increases significantly the ELISA detection sensitivity of the nonstructural-1 glycoprotein in patients infected with DENV type-4
Authors: Lima, Monique da Rocha Queiroz
Nogueira, Rita Maria Ribeiro
Fillipis, Ana Maria Bispo de
Nunes, Priscila Conrado Guerra
Sousa, Carla Santos de
Silva, Manoela Heringer da
Santos, Flavia Barreto dos
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
Abstract: The secreted form of the dengue virus (DENV) nonstructural-1 (NS1) glycoprotein has been shown to be useful for the diagnosis of DENV infections in patients’ serum samples. In a number of studies, the sensitivity of the commercially available DENV NS1 glycoprotein detection assays was higher against some DENV serotypes (DENV-1 > DENV-3 > DENV-2 = DENV-4) than others and were also lower using patients’ serum samples with secondary versus primary DENV infections. In this study, 471 DENV-4 positive acute phase patients’ serum samples were selected from a large panel collected in Brazil from March 2011 to October 2012 by RT-PCR and/or virus isolation followed by serotype determination. The sera from primary (n = 228) and secondary (n = 238) DENV-4 infections were identified using IgM and IgG capture ELISAs. The sensitivity of a commercial DENV NS1 glycoprotein detection ELISA was then assessed when these serum samples were not pre-treated or pre-treated by acid or heat dissociation prior to being tested. Acid and heat dissociation of patients’ serum samples with primary and secondary DENV-4 infections increased significantly the sensitivity of the DENV NS1 glycoprotein detection ELISA from 54.4% to 77.2% (p < 0.05) and 82% (p < 0.05) and from 39.1% to 63.9% (p < 0.05) and 73.1% (p < 0.05), respectively. Treatment of DENV infected patients’ serum samples using simple and rapid heat dissociation step (100 ◦C for 5 min) was, therefore, shown to be very useful for increasing the sensitivity of the DENV NS1 glycoprotein detection ELISA using serum samples from either primary or secondary DENV infected patients.
Keywords: Dengue diagnosis
NS1 ELISA
Immune-complex dissociation
DENV-4
secondary infections
keywords: Dengue
DeCS: Dengue
Diagnóstico
Ensaio de Imunoadsorção Enzimática
Issue Date: 2014
Publisher: Elsevier
Citation: LIMA, Monique da Rocha Queiroz et al. A simple heat dissociation method increases significantly the ELISA detection sensitivity of the nonstructural-1 glycoprotein in patients infected with DENV type-4. Journal of Virological Methods, v. 204, p.105-108, 2014.
DOI: 10.1016/j.jviromet.2014.02.031
ISSN: 0166-0934
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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