Author | Spichler, Anne | |
Author | Ko, Albert Icksang | |
Author | Silva, Everton Fagonde | |
Author | Brito, Thales de | |
Author | Silva, Ana Maria Gonçalves da | |
Author | Athanazio, Daniel Abensur | |
Author | Silva, Cleiton Santos | |
Author | Seguro, Antonio Carlos | |
Access date | 2015-10-26T11:28:01Z | |
Available date | 2015-10-26T11:28:01Z | |
Document date | 2007 | |
Citation | SPICHLER, A. et al. Reversal of renal tubule transporter downregulation during severe leptospirosis with antimicrobial therapy. American Journal of Tropical Medicine and Hygiene, v. 77, n. 6, p. 1111-1119, 2007. | pt_BR |
ISSN | 0002-9637 | |
URI | https://www.arca.fiocruz.br/handle/icict/12080 | |
Language | eng | pt_BR |
Publisher | American Society of Tropical Medicine and Hygiene | pt_BR |
Rights | open access | pt_BR |
Title | Reversal of renal tubule transporter downregulation during severe leptospirosis with antimicrobial therapy. | pt_BR |
Type | Article | pt_BR |
Abstract | Tubular dysfunction is a hallmark of severe leptospirosis. Antimicrobial therapy is thought to interfere on renal involvement. We evaluated the expression of a proximal tubule type-3 Na+/H+ exchanger (NHE3) and a thick ascending limb Na+-K+-2Cl(-) cotransporter (NKCC2) in controls and treated hamsters. Animals infected by a serovar Copenhageni isolate, were treated or not with ampicillin (AMP) and/or N-acetylcysteine (NAC). Leptospiral antigen(s) and expression of renal transporters were evaluated by immunohistochemistry, and serum thiobarbituric acid (TBARS) was quantified. Infected hamsters had high amounts of detectable leptospiral antigen(s) in target tissues while renal expression of NHE3 and NKCC2 decreased. Ampicillin treatment was associated with minimal or no detection of leptospiral antigens, normal expression of NHE3 and NKCC2 transporters, and reduced levels of TBARS. NAC effect was restricted to lowering TBARS. Early and late AMP treatment rescued tubular defects in severe leptospirosis disease, and there was no evidence of benefit from antioxidant therapy. | pt_BR |
Affilliation | University of São Paulo School of Medicine. Faculdade de Medicina USP. Laboratorio Pesquisa Basica LIM/12. Department of Nephrology LIM/12. São Paulo, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | University of Sao Paulo Medical School LIM/06. Institute of Tropical Medicine. Sao Paulo, SP, Brasil | pt_BR |
Affilliation | University of Sao Paulo Medical School LIM/06. Institute of Tropical Medicine. Sao Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | University of São Paulo School of Medicine. Faculdade de Medicina USP. Laboratorio Pesquisa Basica LIM/12. Department of Nephrology LIM/12. São Paulo, SP, Brasil | pt_BR |
DeCS | Ampicilina/farmacologia | pt_BR |
DeCS | Antibacterianos/farmacologia | pt_BR |
DeCS | Regulação da Expressão Gênica/efeitos de drogas | pt_BR |
DeCS | Antiportador de Sódio e Hidrogênio/biossíntese | pt_BR |
DeCS | Simportadores de Cloreto de Sódio-Potássio/biossíntese | pt_BR |
DeCS | Doença de Weil/quimioterapia | pt_BR |
DeCS | Acetilcisteína/administração & dosagem | pt_BR |
DeCS | Ampicilina/administração & dosagem | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Antibacterianos/administração & dosagem | pt_BR |
DeCS | Antígenos de Bactérias/análise | pt_BR |
DeCS | Cricetinae | pt_BR |
DeCS | Depuradores de Radicais Livres/administração & dosagem | pt_BR |
DeCS | Feminino | pt_BR |
DeCS | Perfilação da Expressão Gênica | pt_BR |
DeCS | Rim/patologia | pt_BR |
DeCS | Mesocricetus | pt_BR |
DeCS | Antiportador de Sódio e Hidrogênio/análise | pt_BR |
DeCS | Simportadores de Cloreto de Sódio-Potássio/análise | pt_BR |
DeCS | Tiobarbitúricos/sangue | pt_BR |
DeCS | Doença de Weil/patologia | pt_BR |