Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12080
Title: Reversal of renal tubule transporter downregulation during severe leptospirosis with antimicrobial therapy.
Authors: Spichler, Anne
Ko, Albert Icksang
Silva, Everton Fagonde
Brito, Thales de
Silva, Ana Maria Gonçalves da
Athanazio, Daniel Abensur
Silva, Cleiton Santos
Seguro, Antonio Carlos
Affilliation: University of São Paulo School of Medicine. Faculdade de Medicina USP. Laboratorio Pesquisa Basica LIM/12. Department of Nephrology LIM/12. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
University of Sao Paulo Medical School LIM/06. Institute of Tropical Medicine. Sao Paulo, SP, Brasil
University of Sao Paulo Medical School LIM/06. Institute of Tropical Medicine. Sao Paulo, SP, Brasil
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
University of São Paulo School of Medicine. Faculdade de Medicina USP. Laboratorio Pesquisa Basica LIM/12. Department of Nephrology LIM/12. São Paulo, SP, Brasil
Abstract: Tubular dysfunction is a hallmark of severe leptospirosis. Antimicrobial therapy is thought to interfere on renal involvement. We evaluated the expression of a proximal tubule type-3 Na+/H+ exchanger (NHE3) and a thick ascending limb Na+-K+-2Cl(-) cotransporter (NKCC2) in controls and treated hamsters. Animals infected by a serovar Copenhageni isolate, were treated or not with ampicillin (AMP) and/or N-acetylcysteine (NAC). Leptospiral antigen(s) and expression of renal transporters were evaluated by immunohistochemistry, and serum thiobarbituric acid (TBARS) was quantified. Infected hamsters had high amounts of detectable leptospiral antigen(s) in target tissues while renal expression of NHE3 and NKCC2 decreased. Ampicillin treatment was associated with minimal or no detection of leptospiral antigens, normal expression of NHE3 and NKCC2 transporters, and reduced levels of TBARS. NAC effect was restricted to lowering TBARS. Early and late AMP treatment rescued tubular defects in severe leptospirosis disease, and there was no evidence of benefit from antioxidant therapy.
DeCS: Ampicilina/farmacologia
Antibacterianos/farmacologia
Regulação da Expressão Gênica/efeitos de drogas
Antiportador de Sódio e Hidrogênio/biossíntese
Simportadores de Cloreto de Sódio-Potássio/biossíntese
Doença de Weil/quimioterapia
Acetilcisteína/administração & dosagem
Ampicilina/administração & dosagem
Animais
Antibacterianos/administração & dosagem
Antígenos de Bactérias/análise
Cricetinae
Depuradores de Radicais Livres/administração & dosagem
Feminino
Perfilação da Expressão Gênica
Rim/patologia
Mesocricetus
Antiportador de Sódio e Hidrogênio/análise
Simportadores de Cloreto de Sódio-Potássio/análise
Tiobarbitúricos/sangue
Doença de Weil/patologia
Issue Date: 2007
Publisher: American Society of Tropical Medicine and Hygiene
Citation: SPICHLER, A. et al. Reversal of renal tubule transporter downregulation during severe leptospirosis with antimicrobial therapy. American Journal of Tropical Medicine and Hygiene, v. 77, n. 6, p. 1111-1119, 2007.
ISSN: 0002-9637
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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