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AutorPaiva, Ligia Almeida
AutorCoelho, Karen Almeida
AutorGomes, Tatiana Luna
AutorEl-Cheikh, Marcia Cury
AutorBorojevic, Radovan
AutorPerez, Sandra Aurora
AutorBozza, Patrícia T.
AutorMelo, Christianne Bandeira
Fecha de acceso2015-11-03T14:46:52Z
Fecha de disponibilización2016-04-01T07:30:09Z
Fecha de publicación2015
ReferenciaPAIVA, Ligia Almeida; et al. Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production. Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA), v.95, p.57-62, Apr. 2015.pt_BR
ISSN0952-3278
URIhttps://www.arca.fiocruz.br/handle/icict/12108
Idiomaengpt_BR
EditorElsevierpt_BR
Derechos de autoropen access
TítuloSchistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF productionpt_BR
Tipo del documentoArticle
DOI10.1016/j.plefa.2015.01.004
Resumen en InglésHepatic Stellate Cells (HSCs) play a crucial role in pathogenesis of liver inflammation and fibrosis. During chronic liver injury, HSCs lose vitamin A and transform into myofibroblastic cells. In schistosomal granulomas, these activated HSCs are called GR-HSCs. Schistosomal-triggered hepatic fibrogenesis has TGF-β as the most potent fibrogenic stimulus, that also controls gene expression of the angiogenic molecule VEGF in HSCs. COX-dependent production of prostaglandins (PGs) also play role in angiogenic processes. Besides angiogenic roles, prostanoids control immunomodulation of Schistosoma mansoni infection. Specifically, schistosoma-derived PGD2 has emerged as a key parasite regulator of immune defense evasion, while no role is still established to host PGD2. Therefore, the aim of this work is to investigate the ability of GR-HSCs to synthesize COX-derived PGD2 and a potential role of this prostanoid in VEGF production by GR-HSCs in vitro. Here, we confirmed that GR-HSCs express COX-2, which displayed perinuclear localization. While unstimulated GR-HSCs produce basal levels of PGD2, TGF-β stimulation besides increasing COX2- mRNA levels, enhanced synthesis/secretion of PGD2 in GR-HSCs supernatant. Moreover, GR-HSCs-derived PGD2 mediate VEGF production by TGF-β-stimulated GR-HSCs, since the pre-treatment with HQL-79, an inhibitor of hematopoietic PGD synthase inhibited both PGD2 synthesis and VEGF secretion by TGF-β-stimulated GR-HSCs. All together, our findings show an autocrine/paracrine activity of GR-HSCs-derived PGD2 on TGF-β-induced VEGF production by GR-HSCs, unveiling a role for PGD2 as important regulator of HSCs activation in hepatic granulomas from schistosome infected mice.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónUniversidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónUniversidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.pt_BR
Palavras clave en InglêsHepatic Stellate Cellspt_BR
Palavras clave en InglêsProstaglandin D2pt_BR
Palavras clave en InglêsS. mansonipt_BR
Palavras clave en InglêsVEGFpt_BR
Palavras clave en InglêsEosinophilpt_BR
DeCSCélulas Estreladas do Fígadopt_BR
DeCSProstaglandina D2pt_BR
DeCSEosinófilopt_BR
DeCSSchistosoma mansonipt_BR
Fecha de embargo2016-03-31


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