Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12126
Title: Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry
Authors: Lima, Estela de Oliveira
Macedo, Cristiana Santos de
Esteves, Cibele Zanardi
Oliveira, Diogo Noin de
Pessolani, Maria Cristina Vidal
Nery, José Augusto da Costa
Sarno, Euzenir Nunes
Catharino, Rodrigo Ramos
Affilliation: Universidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.
Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnólogico em Saúde (CDTS). Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Universidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.
Universidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Ambulatório Souza Araújo. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Universidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.
Abstract: Leprosy is a chronic infectious disease caused by Mycobacterium leprae, which primarily infects macrophages and Schwann cells, affecting skin and peripheral nerves. Clinically, the most common form of identification is through the observation of anesthetic lesions on skin; however, up to 30% of infected patients may not present this clinical manifestation. Currently, the gold standard diagnostic test for leprosy is based on skin lesion biopsy, which is invasive and presents low sensibility for suspect cases. Therefore, the development of a fast, sensible and noninvasive method that identifies infected patients would be helpful for assertive diagnosis. The aim of this work was to identify lipid markers in leprosy patients directly from skin imprints, using a mass spectrometric analytical strategy. For skin imprint samples, a 1 cm2 silica plate was gently pressed against the skin of patients or healthy volunteers. Imprinted silica lipids were extracted and submitted to direct-infusion electrospray ionization high-resolution mass spectrometry (ESI-HRMS). All samples were differentiated using a lipidomics-based data workup employing multivariate data analysis, which helped electing different lipid markers, for example, mycobacterial mycolic acids, inflammatory and apoptotic molecules were identified as leprosy patients’ markers. Otherwise, phospholipids and gangliosides were pointed as healthy volunteers’ skin lipid markers, according to normal skin composition. Results indicate that silica plate skin imprinting associated with ESI-HRMS is a promising fast and sensible leprosy diagnostic method. With a prompt leprosy diagnosis, an early and effective treatment could be feasible and thus the chain of leprosy transmission could be abbreviated.
Keywords: Leprosy
High-Resolution Electrospray Mass Spectrometry (ESIHRMS)
Skin Imprinting in Silica Plates
Potential Diagnostic Methodology for Leprosy
keywords: Metodologia de diagnóstico potencial para Hanseníase
DeCS: Hanseníase
Espectrometria de Massas
Placas de sílica
Issue Date: 2015
Publisher: American Chemical Society
Citation: LIMA, Estela de Oliveira; et al. Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry. Analytical Chemistry, v.87, p.3585−3592, 2015.
DOI: 10.1021/acs.analchem.5b00097
ISSN: 0003-2700
Copyright: restricted access
Appears in Collections:CDTS - Artigos de Periódicos
IOC - Artigos de Periódicos

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