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THE OMPA-LIKE PROTEIN LOA22 IS ESSENTIAL FOR LEPTOSPIRAL VIRULENCE
Ristow, Paula et al. | Date Issued: 2007
Author
Ristow, Paula
Bourhy, Pascale
McBride, Flávia Weykamp da Cruz
Figueira, Cláudio Pereira
Huerre, Michel
Ave, Patrick
Girons, Isabelle Saint
Ko, Albert Icksang
Picardeau, Mathieu
Affilliation
Institut Pasteur. Unité de Biologie des Spirochètes. Paris, France / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Goes. Rio de Janeiro, RJ, Brasil
Institut Pasteur. Unité de Biologie des Spirochètes. Paris, France
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Institut Pasteur. Unité de Recherche et d’Expertise Histotechnologie et Pathologie. Paris, France
Institut Pasteur. Unité de Recherche et d’Expertise Histotechnologie et Pathologie. Paris, France
Institut Pasteur. Unité de Biologie des Spirochètes. Paris, France
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. New York, New York, USA
Institut Pasteur. Unité de Biologie des Spirochètes. Paris, France
Abstract
Pathogenic mechanisms of Leptospira interrogans, the causal agent of leptospirosis, remain largely unknown. This is mainly due to the lack of tools for genetic manipulations of pathogenic species. In this study, we characterized a mutant obtained by insertion of the transposon Himar1 into a gene encoding a putative lipoprotein, Loa22, which has a predicted OmpA domain based on sequence identity. The resulting mutant did not express Loa22 and was attenuated in virulence in the guinea pig and hamster models of leptospirosis, whereas the genetically complemented strain was restored in Loa22 expression and virulence. Our results show that Loa22 was expressed during host infection and exposed on the cell surface. Loa22 is therefore necessary for virulence of L. interrogans in the animal model and represents, to our knowledge, the first genetically defined virulence factor in Leptospira species
Keywords in Portuguese
Leptospirose
Leptospira
Leptospira interrogans
Virulência
Animal
Hamaster
 
Publisher
Public Library of Science
Citation
RISTOW, P. et al. The OmpA-Like protein Loa22 is essential for Leptospiral virulence. Plos Pathogens, v. 3, n. 7, p. e97, 2007.
DOI
10.1371/journal.ppat.0030097
ISSN
1553-7366