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T-CELL RECEPTOR Vβ REPERTOIRE OF CD8+ T-LYMPHOCYTE SUBPOPULATIONS IN CUTANEOUS LEISHMANIASIS PATIENTS FROM THE STATE OF RIO DE JANEIRO, BRAZIL
Human cutaneous leishmaniasis
Flow cytometry
CD8+ T-lymphocyte
Leishmania braziliensis
TCR
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Abstract
In human cutaneous leishmaniasis (CL), the immune response is mainly mediated by T-cells. The role of CD8+ Tlymphocytes,
which are related to healing or deleterious functions, in affecting clinical outcome is controversial. The
aim of this study was to evaluate T-cell receptor diversity in late-differentiated effector (LDE) and memory CD8+ T-cell
subsets in order to create a profile of specific clones engaged in deleterious or protective CL immune responses. Healthy
subjects, patients with active disease (PAD) and clinically cured patients were enrolled in the study. Total CD8+ T-lymphocytes
showed a disturbance in the expression of the Vβ2, Vβ9, Vβ13.2, Vβ18 and Vβ23 families. The analyses of CD8+
T-lymphocyte subsets showed high frequencies of LDE CD8+ T-lymphocytes expressing Vβ12 and Vβ22 in PAD, as well as
effector-memory CD8+ T-cells expressing Vβ22. We also observed low frequencies of effector and central-memory CD8+
T-cells expressing Vβ2 in PAD, which correlated with a greater lesion size. Particular Vβ expansions point to CD8+ T-cell
clones that are selected during CL immune responses, suggesting that CD8+ T-lymphocytes expressing Vβ12 or Vβ22 are
involved in a LDE response and that Vβ2 contractions in memory CD8+ T-cells are associated with larger lesions.
Keywords
Vβ repertoireHuman cutaneous leishmaniasis
Flow cytometry
CD8+ T-lymphocyte
Leishmania braziliensis
TCR
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