Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12272
Title: Unveiling the Trypanosoma cruzi Nuclear Proteome
Authors: Santos Júnior, Agenor de Castro Moreira dos
Kalume, Dário Eluan
Camargo, Ricardo
Gómez-Mendoza, Diana Paola
Correa, José Raimundo
Chameau, Sébastien
Sousa, Marcelo Valle de
Lima, Beatriz Dolabela de
Ricart, Carlos André Ornelas
Affilliation: University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinas de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.
University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
Abstract: Replication of Trypanosoma cruzi, the etiological agent of Chagas disease, displays peculiar features, such as absence of chromosome condensation and closed mitosis. Although previous proteome and subproteome analyses of T. cruzi have been carried out, the nuclear subproteome of this protozoan has not been described. Here, we report, for the first time to the best of our knowledge, the isolation and proteome analysis of T. cruzi nuclear fraction. For that, T. cruzi epimastigote cells were lysed and subjected to cell fractionation using two steps of sucrose density gradient centrifugation. The purity of the nuclear fraction was confirmed by phase contrast and fluorescence microscopy. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) allowed the identification of 864 proteins. Among those, 272 proteins were annotated as putative uncharacterized, and 275 had not been previously reported on global T. cruzi proteome analysis. Additionally, to support our enrichment method, bioinformatics analysis in DAVID was carried out. It grouped the nuclear proteins in 65 gene clusters, wherein the clusters with the highest enrichment scores harbor members with chromatin organization and DNA binding functions.
Keywords: Trypanosoma cruzi
Chagas Disease
Nucelar Proteome
DeCS: Trypanosoma cruzi
Doença de Chagas
Proteoma
Issue Date: 2015
Publisher: Plos One
Citation: SANTOS JÚNIOR, Agenor de Castro Moreira dos; et al. Unveiling the Trypanosoma cruzi Nuclear Proteome. Plos One, v.10, n.9, e0138667, 12p, Sept. 2015.
DOI: 10.1371/journal.pone.0138667
ISSN: 1932-6203
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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