The study investigated the profile of use of internet tools by in-service biology teachers in the State of Rio de Janeiro. We identified four purposes of use: Study, Didactic, Professional Management and Personal Socialization. The pedagogical use internet tools proved to be small. It was mostly focused to the search for information about biological contents for individual use and to the download of materials to distribute to the students. We discussed the importance of articulating policies and programs of teacher education through the development of activities to raise awareness about the pedagogical potential of collaborative internet tools among Brazilian teachers.