Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12546
Title: Successful isolation of Leishmania infantum from Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) collected from naturally infected dogs
Authors: Medeiros-Silva, Viviane
Gurgel-Gonçalves, Rodrigo
Nitz, Nadjar
Morales, Lucia Emilia D’ Anduraim
Cruz, Laurício Monteiro
Sobral, Isabele Gonçalves
Boité, Mariana Côrtes
Ferreira, Gabriel Eduardo Melim
Cupolillo, Elisa
Romero, Gustavo Adolfo Sierra
Affilliation: Universidade de Brasília. Programa de Pós-Graduação em Medicina Tropical. Brasília, DF, Brazil.
Universidade de Brasília. Programa de Pós-Graduação em Medicina Tropical. Brasília, DF, Brazil. / Universidade de Brasília. Laboratório de Parasitologia Médica e Biologia de Vetores. Brasília, DF, Brazil.
Universidade de Brasília. Programa de Pós-Graduação em Medicina Tropical. Brasília, DF, Brazil.
Diretoria de Vigilância Ambiental do Distrito Federal, Brasília, DF, Brazil.
Diretoria de Vigilância Ambiental do Distrito Federal, Brasília, DF, Brazil.
Diretoria de Vigilância Ambiental do Distrito Federal, Brasília, DF, Brazil.
Fundação Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brazil.
Universidade de Brasília. Programa de Pós-Graduação em Medicina Tropical. Brasília, DF, Brazil. / Universidade de Brasília. Laboratório de Leishmanioses. Núcleo de Medicina Tropical. Brasília, DF, Brazil.
Abstract: Background: The main transmission route of Leishmania infantum is through the bites of sand flies. However, alternative mechanisms are being investigated, such as through the bites of ticks, which could have epidemiological relevance. The objective of this work was to verify the presence of Leishmania spp. in Rhipicephalus sanguineus sensu lato collected from naturally infected dogs in the Federal District of Brazil. Methods: Ticks were dissected to remove their intestines and salivary glands for DNA extraction and the subsequent amplification of the conserved region of 120 bp of kDNA and 234 bp of the hsp70 gene of Leishmania spp. The amplified kDNA products were digested with endonucleases HaeIII and BstUI and were submitted to DNA sequencing. Isolated Leishmania parasites from these ticks were analyzed by multilocus enzyme electrophoresis, and the DNA obtained from this culture was subjected to microsatellite analyses. Results: Overall, 130 specimens of R. sanguineus were collected from 27 dogs. Leishmania spp. were successfully isolated in culture from five pools of salivary glands and the intestines of ticks collected from four dogs. The amplified kDNA products from the dog blood samples and from the tick cultures, when digested by HaeIII and BstUI, revealed the presence of L. braziliensis and L. infantum. One strain was cultivated and characterized as L. infantum by enzyme electrophoresis. The amplified kDNA products from the blood of one dog showed a sequence homology with L. braziliensis; however, the amplified kDNA from the ticks collected from this dog showed a sequence homology to L. infantum. Conclusion: The results confirm that the specimens of R. sanguineus that feed on dogs naturally infected by L. infantum contain the parasite DNA in their intestines and salivary glands, and viable L. infantum can be successfully isolated from these ectoparasites.
Keywords: Leishmania infantum
Rhipicephalus sanguineus
PCR
Canis familiaris
Issue Date: 2015
Publisher: BMC Veterinary Research
Citation: Medeiros-Silva, V., et al. Successful isolation of Leishmania infantum from Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) collected from naturally infected dogs. BMC Veterinary Research. 11:258, 2015.
DOI: DOI 10.1186/s12917-015-0576-5
Copyright: open access
Appears in Collections:RO - Artigos de Periódicos

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