Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12625
Title: Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus
Authors: Lima Junior, Raimundo Sousa
Mello, Cintia da Silva
Siani, Antonio Carlos
Valente, Ligia M. Marino
Kubelka, Claire Fernandes
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia de Fármacos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.
Abstract: Dengue is the major arbovirus in the world annually causing morbidity and death. The severe dengue is associated with changes on the endothelial barrier function due to the production of inflammatory mediators by immune cells and by the endothelium. Dengue virus (DENV) replicates efficiently in human endothelial cells in vitro and elicits immune responses resulting in endothelial permeability. Uncaria tomentosa (Willd.) DC.(Rubiaceae), known as cat’s claw, has been used in folk medicine for the treatment of a wide-array of symptoms and several scientific studies reported its antiviral, immunomodulatory, anti-inflammatory and antioxidant properties. Here we infected a human lineage of dermal microvascular endothelial cells (HMEC-1) with DENV-2 and treated it with an alkaloidal fraction from U. tomentosa bark (AFUT). We showed antiviral and immunomodulatory activities of U. tomentosa by determining the NS1 antigen and IL-8 in supernatant of DENV-2 infected HMEC-1. Furthermore, by measurement of transendothelial electrical resistance (TEER) we demonstrated for the first time that a plant derivative contributed to reduce paracellular permeability in DENV-2 infected HMEC-1. We also showed that IL-8 contributed significantly to the induction of permeability. Further studies are still required until a new drug can be indicated, but our in vitro results support the evidences that AFUT could be potentially useful to preventive treatment of severe dengue.
Keywords: Uncaria tomentosa
Dengue
Antiviral
Oxindole alkaloid
Endothelial cells
Permeability
DeCS: Células Endoteliais
Unha-de-Gato
Dengue
Antivirais
Permeability
Issue Date: 2013
Publisher: Natural Product Communications
Citation: LIMA JUNIOR, Raimundo Sousa; et al. Uncaria tomentosa alkaloidal fraction reduces paracellular permeability, IL-8 and NS1 production on human microvascular endothelial cells infected with Dengue virus. Natural Product Comuniations, v.8, n.0, 4p, 2013.
ISSN: 1555-9475
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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