Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/13111
Title: Contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from Rio de Janeiro: implications for vector control
Authors: Rasic, Gordana
Schama, Renata
Powell, Rossana
Freitas, Rafael Maciel de
Endersby-Harshman, Nancy M.
Filipovic, Igor
Sylvestre, Gabriel
Máspero, Renato C.
Hoffmann, Ary A.
Affilliation: The University of Melbourne. Bio21 Institute. School of Biosciences. Pest and Environmental Adaptation Research Group. Parkville, Vic, Australia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil.
The University of Melbourne. Bio21 Institute. School of Biosciences. Pest and Environmental Adaptation Research Group. Parkville, Vic, Australia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
The University of Melbourne. Bio21 Institute. School of Biosciences. Pest and Environmental Adaptation Research Group. Parkville, Vic, Australia.
The University of Melbourne. Bio21 Institute. School of Biosciences. Pest and Environmental Adaptation Research Group. Parkville, Vic, Australia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Secretaria Municipal de Saúde do Rio de Janeiro. Superintendência de Vigilância em Saúde. Coordenação de Vigilância Ambiental em Saúde. Gerência de Risco Biológico. Rio de Janeiro, RJ, Brasil.
The University of Melbourne. Bio21 Institute. School of Biosciences. Pest and Environmental Adaptation Research Group. Parkville, Vic, Australia.
Abstract: Dengue is the most prevalent global arboviral disease that affects over 300 million people every year. Brazil has the highest number of dengue cases in the world, with the most severe epidemics in the city of Rio de Janeiro (Rio). The effective control of dengue is critically dependent on the knowledge of population genetic structuring in the primary dengue vector, the mosquito Aedes aegypti. We analyzed mitochondrial and nuclear genomewide single nucleotide polymorphism markers generated via Restriction-site Associated DNA sequencing, as well as traditional microsatellite markers in Ae. aegypti from Rio. We found four divergent mitochondrial lineages and a strong spatial structuring of mitochondrial variation, in contrast to the overall nuclear homogeneity across Rio. Despite a low overall differentiation in the nuclear genome, we detected strong spatial structure for variation in over 20 genes that have a significantly altered expression in response to insecticides, xenobiotics, and pathogens, including the novel biocontrol agent Wolbachia. Our results indicate that high genetic diversity, spatially unconstrained admixing likely mediated by male dispersal, along with locally heterogeneous genetic variation that could affect insecticide resistance and mosquito vectorial capacity, set limits to the effectiveness of measures to control dengue fever in Rio.
Keywords: Aedes aegypti
RAD-seq
Rio de Janeiro
Genetic structure
Microsatellites
Mito-nuclear discordance
Vector control
DeCS: Aedes
Controle de Vetores
Issue Date: 2015
Publisher: open access
Citation: RASIC, Gordana; et al. Contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from Rio de Janeiro: implications for vector control. Evolutionary Applications, v.8, n.9, p.901-15, Oct. 2015.
DOI: 10.1111/eva.12301
ISSN: 1752-4571
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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