Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/13194
Title: Detection of Common, Emerging and Uncommon VP4, and VP7 Human Group A Rotavirus Genotypes from Urban Sewage Samples in Uruguay
Authors: Tort, Luis Fernando Lopez
Victoria, Matías
Lizasoain, Andrés
García, Mariana
Berois, Mabel
Cristina, Juan
Leite, José Paulo Gagliardi
Gómez, Mariela Martínez
Miagostovich, Marize Pereira
Colina, Rodney
Affilliation: Universidad de la República. Departamento de Ciencias Biológicas, Regional Norte – CENUR Litoral Norte. Laboratorio de Virología Molecular. Salto, Uruguay.
Universidad de la República. Departamento de Ciencias Biológicas, Regional Norte – CENUR Litoral Norte. Laboratorio de Virología Molecular. Salto, Uruguay.
Universidad de la República. Departamento de Ciencias Biológicas, Regional Norte – CENUR Litoral Norte. Laboratorio de Virología Molecular. Salto, Uruguay.
Universidad de la República. Departamento de Ciencias Biológicas, Regional Norte – CENUR Litoral Norte. Laboratorio de Virología Molecular. Salto, Uruguay.
Universidad de la República. Facultad de Ciencias. Sección Virologia. Montevideo, Uruguay.
Universidad de la República. Facultad de Ciencias. Centro de Investigaciones Nucleares. Laboratorio de Virología Molecular. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Universidad de la República. Departamento de Ciencias Biológicas, Regional Norte – CENUR Litoral Norte. Laboratorio de Virología Molecular. Salto, Uruguay.
Abstract: Environmental approach has proven to be a useful tool for epidemiological studies demonstrating through environmental studies the diversity of viruses circulating in a given population. The aim of this study was to perform a phylogenetic characterization of the group A rotavirus (RVA) glycoprotein (G)- and protease-sensitive (P)-genotypes obtained from sewage samples (n = 116) collected in six cities of Uruguay during March 2011 to April 2013. A worldwide standardized semi-nested multiplex RT-PCR (SNM RT-PCR) protocol directed against VP4 and VP7 genes were conducted for RVA detection and consensual DNA fragments were submitted to nucleotide sequencing. P and/or G genotype was successfully determined by phylogenetic analysis in 61 % (n = 37) of the positive samples obtained by SNM RT-PCR (n = 61). The RVA genotypes were as follow: G1 (n = 2), G2 (n = 14), G3 (n = 5), G12 (n = 2), P[4] (n = 4), P[8] (n = 16), and P[3] (n = 2). Interestingly, through phylogenetic analysis, emerging, and uncommon human genotypes could be detected. Results obtained from the comparison of RVA genotypes detected in the current study and Uruguayan RVA strains previously described for contemporary clinical pediatric cases showed that monitoring sewage may be a good screening option for a rapid and economical overview of the circulating genotypes in the surrounding human population and a useful approximation to study RVA epidemiology in a future vaccine monitoring program. The present study represents the first report in Uruguay that describes the phylogenetic diversity of RVA from urban sewage samples.
Keywords: Group A rotavirus
G- and P-types
Phylogeny
Epidemiology
Sewage
Uruguay
keywords: Epidemiologia
Uruguai
Filogenia
Esgotos
Rotavirus
Infecções por Rotavirus
Issue Date: 2015
Publisher: Springer Verlag
Citation: TORT, Luis Fernando Lopes; et al. Detection of Common, Emerging and Uncommon VP4, and VP7 Human Group A Rotavirus Genotypes from Urban Sewage Samples in Uruguay. Food Environ Virol, v.7, n.4, p.342–353, Dec. 2015.
DOI: 10.1007/s12560-015-9213-5
ISSN: 1867-0334
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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