Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/13201
Title: Diversity and molecular characterization of novel hemoplasmas infecting wild rodents from different Brazilian biomes
Authors: Gonçalves, Luiz Ricardo
Roque, André Luiz Rodrigues
Matos, Carlos Antonio
Fernandes, Simone de Jesus
Olmos, Isabella Delamain Fernandez
Machado, Rosangela Zacarias
André, Marcos Rogério
Affilliation: Universidade Estadual Paulista (FCAV/UNESP). Pós-graduação em Microbiologia Agropecuária. Jaboticabal, SP, Brasil / Universidade Estadual Paulista (FCAV/UNESP). Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária. Laboratório de Imunoparasitologia. Jaboticabal, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.
Universidade Estadual Paulista (FCAV/UNESP). Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária. Laboratório de Imunoparasitologia. Jaboticabal, SP, Brasil / Direcção de Ciências Animais. Maputo, Moçambique.
Universidade Estadual Paulista (FCAV/UNESP). Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária. Laboratório de Imunoparasitologia. Jaboticabal, SP, Brasil.
Universidade Estadual Paulista (FCAV/UNESP). Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária. Laboratório de Imunoparasitologia. Jaboticabal, SP, Brasil.
Universidade Estadual Paulista (FCAV/UNESP). Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária. Laboratório de Imunoparasitologia. Jaboticabal, SP, Brasil.
Universidade Estadual Paulista (FCAV/UNESP). Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária. Laboratório de Imunoparasitologia. Jaboticabal, SP, Brasil.
Abstract: Although hemoplasma infection in domestic animals has been well documented, little is known about the prevalence and genetic diversity of these bacteria in wild rodents. The present work aimed to investigate the occurrence of hemotrophic mycoplasmas in wild rodents from five Brazilian biomes, assessing the 16S rRNA phylogenetic position of hemoplasma species by molecular approach. Spleen tissues were obtained from 500 rodents, comprising 52 different rodent species trapped between 2000 and 2011. DNA samples were submitted to previously described PCR protocols for amplifying Mycoplasma spp. based on 16S rRNA, followed by sequencing and phylogenetic inferences. Among 457 rodent spleen samples showing absence of inhibitors, 100 (21.9%) were PCR positive to Mycoplasma spp. The occurrence of hemotropic mycoplasmas among all sampled rodents was demonstrated in all five biomes and ranged from 9.3% (7/75) to 26.2% (38/145). The Blastn analysis showed that amplified sequences had a percentage of identity ranging from 86 to 99% with other murine hemoplasmas. The ML phylogenetic analysis of 16S rRNA gene of 24 positive randomly selected samples showed the presence of ten distinct groups, all clustering within the Mycoplasma haemofelis. The phylogenetic assessment suggests the circulation of novel hemoplasma species in rodents from different biomes in Brazil.
Keywords: Hemotropic mycoplasmas
Wild rodents
Molecular characterization
Brazil
DeCS: Roedores
Brasil
Issue Date: 2015
Publisher: Elsevier
Citation: GONÇALVES, Luiz Ricardo; et al. Diversity and molecular characterization of novel hemoplasmas infecting wild rodents from different Brazilian biomes. Comparative Immunology, Microbiology and Infectious Diseases, v.43, p.50-56, Dec. 2015.
DOI: 10.1016/j.cimid.2015.10.006
ISSN: 0147-9571
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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