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AutorGuedes, Carlos Eduardo Sampaio
AutorLima, José Geraldo Bomfim
AutorHelfer, Emmanuèle
AutorVeras, Patrícia Sampaio Tavares
AutorViallat, Annie
Fecha de acceso2016-03-31T18:38:54Z
Fecha de disponibilización2016-03-31T18:38:54Z
Fecha de publicación2015
ReferenciaGUEDES, C. E. S. et al. Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles. Plos One, v. 10, n. 8, p. e0134925, 2015.pt_BR
ISSN1932-6203
URIhttps://www.arca.fiocruz.br/handle/icict/13430
Idiomaengpt_BR
EditorPublic Library of Sciencept_BR
Derechos de autoropen accesspt_BR
Palabras clave en PortuguésLeishmaniapt_BR
Palabras clave en PortuguésMacrófagospt_BR
Palabras clave en PortuguésLeishmania amazonensispt_BR
Palabras clave en PortuguésMamíferospt_BR
Palabras clave en PortuguésFagocitosept_BR
TítuloEncapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuolespt_BR
Tipo del documentoArticlept_BR
DOI10.1371/journal.pone.0134925
Resumen en InglésAfter phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate. Here, using a variant of the ‘inverted emulsion’ method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs. We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome. L. amazonensis promastigotes encapsulated in liposomes filled with RPMI-Dextran solution at pH 7.5 or 6.5 survived up to 96 h at 24°C. At 37°C and pH 5.5, parasites survived 48h. This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell. This method will also facilitate the study of the temporal evolution of biophysical properties of the PV during its maturation.pt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasilpt_BR
AfiliaciónAix-Marseille Université. CNRS. Marseille, Francept_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasilpt_BR
AfiliaciónAix-Marseille Université. CNRS. Marseille, Francept_BR


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